Protein microcapsules integrated hierarchical scaffolds for local treatment of acute myocardial infarction model
Protein microcapsules integrated hierarchical scaffolds for local treatment of acute myocardial infarction model
复制标题
蛋白质微胶囊集成分层支架局部治疗急性心肌梗死模型
DOI：
10.1016/j.apmt.2020.100901
复制
发表时间：
2021-03-01
影响因子：
8.3
通讯作者：
Jin, Wei
中科院分区：
文献类型：
--
作者：
Huang, Fanyi;Sun, Lingyu;Jin, Wei
急性心肌梗死（acute myocardial infarction，AMI）作为一种严重的心血管疾病，因其高发病率和高死亡率而受到越来越多的关注和研究。本文利用微流控模板设计了一种新型的蛋白质微胶囊集成分级支架，用于治疗AMI模型。基于先进的微流控电喷雾技术，制备了尺寸可控的核壳微球，以构建双重屏障，提高药物的稳定性。这些微球进一步与电纺聚合物膜组合以构建所需的分层结构。基于这些设计，微囊可以实现药物的长期释放，而聚合物膜支架在体外具有抗粘附作用。降低血管紧张素的水平。ACE 2通过抑制RAS通路，增加Ang（1-7）浓度，增强其保护作用。为了评价治疗效果，将ACE 2-修饰的分级支架应用于AMI小鼠模型，其实现了区域持续释放，改善了缺血病变的严重程度，并具有预防术后粘连的额外效用。与心肌内注射相比，植入分级支架避免了过度损伤，显示了更明显的治疗效果。这些性质表明，我们的分层心脏支架提供了一个潜在的局部治疗AMI的药物管理方法。(C)2020爱思唯尔有限公司版权所有。
As a severe cardiovascular disease, acute myocardial infarction (AMI) has been attaching increasing attention and research for its high morbidity and mortality. In this paper, the novel protein microcapsules integrated hierarchical scaffold is designed for treating AMI model by using microfluidic templates. Based on advanced microfluidic electrospray technique, core-shell microspheres with controllable size are fabricated to build a double barrier for improving the stability of encapsulated drug. These microspheres are further combined with an electrospun polymer membrane to construct the desired hierarchical structure. Based on these designs, the microcapsules can realize long-term drug release while the polymer membrane scaffold exhibits anti-adhesive effect in vitro. By decreasing the level of Angiotensin. and inhibiting the Renin-Angiotensin System (RAS) pathway, ACE2 can increase the concentration of Ang (1-7) and enhance its protective function. To evaluate the therapeutic effect, ACE2-encapsulted hierarchical scaffold is applied to AMI mice model, which achieves regional sustainable release and improves the severity of ischemic lesion, with an additional utility of preventing post-operative adhesion. Compared with injecting intramyocardially, implanting hierarchical scaffold avoids excess injury and shows more obvious therapeutic efficacy. These properties suggest that our hierarchical cardiac scaffolds provide a potential drug administration approach for local treatment of AMI. (C) 2020 Elsevier Ltd. All rights reserved.