Differential effects of clathrin and actin inhibitors on internalization of Escherichia coli and Salmonella choleraesuis in porcine jejunal Peyer's patches
Differential effects of clathrin and actin inhibitors on internalization of Escherichia coli and Salmonella choleraesuis in porcine jejunal Peyer's patches
复制标题
DOI：
10.1016/j.vetmic.2005.10.022
复制
发表时间：
2006-03-10
影响因子：
3.3
通讯作者：
Brown, DR
中科院分区：
文献类型：
--
作者：
Green, BT;Brown, DR
派尔集合淋巴结是一个诱导性免疫部位，也是肠道病原体入侵的途径。将猪空肠的派伊尔集合淋巴结粘膜置于Ussing室中，猪霍乱沙门氏菌疫苗株SC-54或非致病性啮齿动物和猪大肠杆菌菌株接触派伊尔集合淋巴结粘膜90 min。通过庆大霉素耐药性测定定量内化细菌。单丹酰尸胺（300 μ M，管腔添加），网格蛋白介导的内吞作用的抑制剂，显着抑制两个E。大肠杆菌菌株相对于未经抑制剂处理的组织; SC-54的内化不受影响。肌动蛋白破坏剂细胞松弛素D（10 μ M，鲁米那添加），抑制内化的猪适应性E。而鼠适应性E. coli或SC-54。SC-54和非致病性E. Peyer结中的大肠杆菌似乎通过不同的细胞途径发生。(C)2005 Elsevier B. V.保留所有权利。
Peyer's patches constitute both an inductive immune site and an enteropathogen invasion route. Peyer's patch mucosae from porcine jejunum were mounted in Ussing chambers, and either Salmonella choleraesuis vaccine strain SC-54 or non-pathogenic rodent and porcine Escherichia coli strains contacted the Peyer's patch mucosa for 90 min. Internalized bacteria were quantified by a gentamicin resistance assay. Monodansylcadaverine (300 mu M, luminal addition), an inhibitor of clathrin-mediated endocytosis, significantly inhibited internalization of both E. coli strains relative to tissues untreated with the inhibitor; internalization of SC-54 was unaffected. The actin-disrupting agent cytochalasin D (10 mu M, luminal addition), inhibited internalization of pig-adapted E. coli but not that of rodent-adapted E. coli or SC-54. Internalization of SC-54 and nonpathogenic E. coli in Peyer's patches appears to occur through different cellular routes. (C) 2005 Elsevier B.V. All rights reserved.