THE 5' ENDS OF LINE1 REPEATS IN RABBIT DNA DEFINE SUBFAMILIES AND REVEAL A SHORT SEQUENCE CONSERVED BETWEEN RABBITS AND HUMANS
THE 5' ENDS OF LINE1 REPEATS IN RABBIT DNA DEFINE SUBFAMILIES AND REVEAL A SHORT SEQUENCE CONSERVED BETWEEN RABBITS AND HUMANS
复制标题
DOI：
10.1016/s0888-7543(05)80222-8
复制
发表时间：
1992-10-01
期刊：
影响因子：
4.4
通讯作者：
HARDISON, RC
中科院分区：
文献类型：
--
作者：
PRICE, DK;AYRES, JA;HARDISON, RC
对兔基因组(L10c)5个全长LINE1(L1)重复序列的5‘端进行了定位，并测定了它们的核苷酸序列。计算机比对表明，这5个L10c重复序列可以分为亚家族，每个亚家族在第一开放阅读框架(ORF1)的上游都有一个特征序列。这5个L1Ocs的大小范围从6.5到7.3kb，5‘端位于ORF1上游76到1125bp。其中两个亚科似乎是从至少6600万年前的一个共同祖先那里分化出来的。对来自兔、人、小鼠和大鼠的L1s的5‘末端的比较表明，除了在所有来自兔和人的特征全长L1s的开头附近发现了一个22bp的序列外，没有发现与ORF1相同的5’端序列。统计分析表明，这一22BP排列的区块具有非常重要的意义。这个22个碱基的部分序列与免疫球蛋白基因增强子中的μE1结合位点相匹配。这一强烈的保守性表明，μE1结合位点可能是兔和人L1重复序列5‘端转录调控元件的一部分。
The 5′ ends of five full-length LINE1 (L1) repeats from the rabbit genome (L1Oc) were mapped and their nucleotide sequences determined. Computer-generated alignments showed that these five L1Oc repeats can be divided into subfamilies, each of which has a characteristic sequence upstream of the first open reading frame (ORF1). These five L1Ocs range in size from 6.5 to 7.3 kb, with 5′ ends located 76 to 1125 bp upstream of ORF1. Two of these subfamilies appear to have diverged from a common ancestor at least 66 million years ago. Comparisons of the 5′ ends of L1s from rabbit, human, mouse, and rat show no common sequence 5′ to ORF1, except for a 22-bp sequence that is found near the beginning of all characterized full-length L1s from rabbit and human. A statistical analysis indicates that this 22-bp aligned block is highly significant. Part of this 22-bp sequence matches theμE1 binding site in immunoglobulin gene enhancers. This strong conservation suggests that theμE1 binding site may be part of a transcriptional regulatory element at the 5′ ends of rabbit and human L1 repeats.