Transcripts from the adenovirus-2 major late promoter yield a single early family of 3′ coterminal mRNAs and five late families
Transcripts from the adenovirus-2 major late promoter yield a single early family of 3′ coterminal mRNAs and five late families
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腺病毒 2 主要晚期启动子的转录物产生 3 共端 mRNA 的单个早期家族和五个晚期家族
DOI：
10.1016/0092-8674(80)90568-1
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发表时间：
1980
期刊：
影响因子：
64.5
通讯作者：
E. Ziff
中科院分区：
文献类型：
--
作者：
A. Shaw;E. Ziff
腺病毒-2的主要晚期启动子位于坐标16.45，启动核前体的合成，这些前体被加工成mrna，这些mrna属于5个3 '共末端家族，Ll-L5。这些mrna都有一个共同的3 - 5 '先导体，在RNA起始位点有一个编码的带帽末端。我们发现，16.45 RNA起始位点也是一个早期启动子，在感染后1小时，在DNA复制和6-8小时的早期开关之前，产生可检测的转录本。来自第一个3 '共末端家族的多聚腺苷化细胞质RNA， Ll，也在感染的最初阶段产生。在环己亚胺存在的情况下，Ll mRNA在细胞质中积累，从而阻止DNA复制和晚期的开始。早期核RNA含有与晚期转录本相同的带帽5 '末端RNAase Tl非核核苷酸和启动子近端寡核苷酸。这意味着同样的转录起始站点用于早期将信使rna synthesls末末阶段用于Ll-L5信使rna合成。与Ll mRNA的早期出现相反，直到感染后5-6小时才检测到L2和L3 mRNA。我们得出结论，Ad-2早-晚开关中的一个主要事件是激活L2-L5 mRNA产生的一种新的控制形式。
The major late promoter of adenovirus-2 is located at coordinate 16.45 and initiates synthesis of nuclear precursors that are processed into mRNAs which fall into five 3’co-terminal families, Ll-L5. These mRNAs all share a common tripartite 5’leader with a capped terminus encoded at the RNA initiation site. We show that the coordinate 16.45 RNA initiation site Is also an early promoter, and yields transcripts detectable as early as 1 hr postinfection, prior to DNA replication and the earlylate switch at 6-8 hr. Polyadenylated cytoplasmic RNA from the first 3’co-terminal family, Ll, is also produced from the earliest stages of infection. Ll mRNA accumulates in the cytoplasm in the presence of cycloheximide, which blocks DNA replication and the onset of the late phase. Early nuclear RNA contains the same capped 5’terminal RNAase Tl undecanucleotide and promoter proximal oligonucleotides present in late transcripts. This implies that precisely the same transcription start site is utilized for early Ll mRNA synthesls as is used during the late stage for Ll-L5 late mRNA synthesis. In contrast to the early appearance of Ll mRNA, neither L2 nor L3 mRNAs are detected until 5-6 hr post infection. We conclude that a major event in the Ad-2 early-late switch is a novel form of control which activates L2-L5 mRNA production.