A DIRECT CORRELATION BETWEEN THE LEVELS OF ASCORBIC-ACID AND H2O2 IN AQUEOUS-HUMOR
A DIRECT CORRELATION BETWEEN THE LEVELS OF ASCORBIC-ACID AND H2O2 IN AQUEOUS-HUMOR
复制标题
DOI：
10.1016/0014-4835(84)90142-8
复制
发表时间：
1984-01-01
影响因子：
3.4
通讯作者：
REDDY, VN
中科院分区：
文献类型：
--
作者：
GIBLIN, FJ;MCCREADY, JP;REDDY, VN
有证据表明，H2 O2存在于房水中产生的抗坏血酸，这也是存在于这种流体，但过氧化物的程度是从抗坏血酸衍生的是未知的。H2 O2和抗坏血酸的浓度，通常存在于各种物种的房水中，也在实验改变流体中的抗坏血酸水平的条件下，进行了测量。家兔和豚鼠眼房水中抗坏血酸的浓度比大鼠和青蛙眼房水中抗坏血酸的浓度高10倍。H_2O_2在家兔和豚鼠眼内的浓度是在大鼠和青蛙眼内的4-10倍。与兔后房中抗坏血酸的浓度高于前房中抗坏血酸的浓度一致，后房中H2 O2的浓度也显著较高。当兔眼房水中的抗坏血酸通过腹膜内给予该化合物而升高时，前部和后部眼房水中的H2 O2水平均显著增加。当通过将豚鼠置于抗坏血酸缺乏的饮食上来实验性地降低抗坏血酸水平时，在眼房水中观察到抗坏血酸和H2 O2两者的水平降低10倍。在使动物恢复正常饮食后，两种化合物的浓度均恢复至对照值。抗坏血酸和H2 O2在房水中的浓度之间的直接相关性表明，抗坏血酸是H2 O2在这种流体中的主要来源。
There is evidence that H2O2 present in aqueous humor arises from ascorbic acid which is also present in this fluid, but the extent to which peroxide is derived from ascorbic acid is not known. The concentrations of H2O2 and ascorbic acid, normally present in the aqueous humor of various species and also under conditions in which the level of ascorbic acid in the fluid was experimentally altered, were measured. In aqueous humor of rabbit and guinea pig the concentration of ascorbic acid was 10 times higher than that present in aqueous of rat and frog. The concentration of H2O2 was 4-10 times higher in rabbit and guinea pig aqueous compared to that in rat and frog. Consistent with the higher concentration of ascrobic acid in posterior compared to anterior aqueous humor in the rabbit, the concentration of H2O2 was also significantly higher in the posterior aqueous. When ascorbic acid in rabbit aqueous humor was elevated by i.p. administration of the compound, there was a significant increase in the level of H2O2 in both anterior and posterior aqueous humor. When the level of ascorbic acid was lowered experimentally by placing guinea pigs on an ascorbic acid deficient diet, a 10-fold decrease in the level of boht ascorbic acid and H2O2 was observed in the aqueous humor. Upon returning the animals to a normal diet, the concentrations of both compounds returned to control values. The direct correlation between the concentrations of ascorbic acid and H2O2 in aqueous humor suggests that ascrobic acid is the primary source of H2O2 in this fluid.