DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES - MORPHOLOGY CYTOCHEMISTRY AND BIOCHEMISTRY
DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES - MORPHOLOGY CYTOCHEMISTRY AND BIOCHEMISTRY
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DOI：
10.1084/jem.121.1.153
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发表时间：
1965-01-01
影响因子：
15.3
通讯作者：
BENSON, B
中科院分区：
文献类型：
--
作者：
COHN, ZA;BENSON, B
描述了从未受刺激的小鼠腹膜腔中同质的单核细胞样细胞群的体外分化。在所采用的条件下，细胞大小的逐渐增加没有显著的细胞分裂。这一过程的形态学特征是相密集和中性红阳性颗粒、线粒体和脂滴的积累。相密颗粒对酸性磷酸酶反应强烈。生化测定表明，酸性磷酸酶、组织蛋白酶和/3-葡萄糖醛酸酶的总含量和比活性显著增加。酸性磷酸酶的产生比其他两种水解酶更为迅速和广泛。从这些数据来看，单核细胞样细胞向成熟巨噬细胞的转化似乎伴随着溶酶体样细胞器数量增加的形成。用细菌脂多糖刺激小鼠腹膜吞噬细胞在体内经历一系列类似的形态和生化事件。
The in vitro differentiation of homogeneous populations of monocyte-like cells from the unstimulated mouse peritoneal cavity is described. Under the conditions employed, a progressive increase in cell size occurs without significant cell division. This process is characterized morphologically by the accumulation of phase-dense and neutral red-positive granules, mitochondria, and lipid droplets. The phase-dense granules react strongly for acid phosphatase. Biochemical determinations indicate marked increases in the total content and specific activity of acid phosphatase, cathepsin, and /3-glucuroni-dase. The production of acid phosphatase is more rapid and extensive than that of the other 2 hydrolases. From these data it appears that the conversion of a monocyte-like cell to a mature macrophage is accompanied by the formation of increased numbers of lysosome- like cytoplasmic organelles. Mouse peritoneal phagocytes stimulated in vivo with a bacterial lipopolysaccharide undergo a similar series of morphological and biochemical events.