Ischemic preconditioning of the rat brain as a method of endothelial protection from ischemic/repercussion injury.
Ischemic preconditioning of the rat brain as a method of endothelial protection from ischemic/repercussion injury.
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DOI：
10.1007/s11055-005-0095-0
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发表时间：
2005-07-01
影响因子：
--
通讯作者：
Polyakova, E A
中科院分区：
文献类型：
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作者：
Vlasov, T D;Korzhevskii, D E;Polyakova, E A
这些研究报道了局部和远程脑缺血预适应对大鼠脑内皮细胞的保护作用。采用椎动脉热凝结合夹闭颈动脉的方法复制持续30min的脑缺血模型，再经颈动脉再灌注120min(对照组)。复制缺血预适应和远距离预适应的早期和晚期。用高频多普勒超声仪记录脑血流量。局部缺血预适应的早期和晚期以及远程缺血预适应的晚期具有明显的内皮保护作用，表现为改善缺血后大鼠脑血流的恢复，内皮脱屑和脑水肿程度较低。阻断一氧化氮合成可消除预适应的两个阶段的保护作用。
The studies reported here addressed the endothelium-protecting action of local and remote ischemic preconditioning of the brain in rats. Cerebral ischemia lasting 30 min was reproduced by thermocoagulation of the vertebral arteries with simultaneous clamping of the carotid arteries, the procedure being followed by reperfusion via the carotid arteries for 120 min (controls). The early and late phases of ischemic preconditioning and remote preconditioning were reproduced. Brain blood flow was recorded using high-frequency Doppler ultrasonography. The early and late phases of local ischemic preconditioning and the late phase of remote ischemic preconditioning were found to have endothelium-protecting actions apparent as improvements in the recovery of brain blood flow in the post-ischemic period in preconditioned rats, with lower levels of endothelial desquamation and cerebral edema. Blockade of nitric oxide synthesis eliminated the protective effects of both phases of preconditioning.