Overcoming the effects of matrix interference in the measurement of urine protein analytes.
Overcoming the effects of matrix interference in the measurement of urine protein analytes.
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DOI：
10.4137/bmi.s8703
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发表时间：
2012
影响因子：
3.8
通讯作者：
Oates JC
中科院分区：
文献类型：
--
作者：
Taylor TP;Janech MG;Slate EH;Lewis EC;Arthur JM;Oates JC
使用多重头测定法测量尿蛋白具有发现生物标志物的巨大潜力，但尿液中的物质（基质）会干扰测定结果。通过与几种已知数量的常见分析物进行比较，本研究表明尿液基质对低丰度蛋白质的准确测量有不同的干扰。稀释尿液可以更准确地测量这些蛋白质，相当于稀释后的分析物超过检测限制时的标准稀释技术。因此，稀释可以作为尿液免疫测定中克服尿基质效应的有效技术。这些结果可能适用于基质成分干扰测定性能的其他生物液体。
Using multiplex bead assays to measure urine proteins has a great potential for biomarker discovery, but substances in urine (the matrix) can interfere with assay measurements. By comparing the recovery of urine spiked with known quantities of several common analytes, this study demonstrated that the urine matrix variably interfered with the accurate measurement of low abundance proteins. Dilution of the urine permitted a more accurate measure of these proteins, equivalent to the standard dilution technique when the diluted analytes were above the limits of detection of the assay. Therefore, dilution can be used as an effective technique for over-coming urine matrix effects in urine immunoassays. These results may be applicable to other biological fluids in which matrix components interfere with assay performance.