IMMUNOLOGICAL PROPERTIES OF RICKETTSIA-TSUTSUGAMUSHI, KAWASAKI STRAIN, ISOLATED FROM A PATIENT IN KYUSHU
IMMUNOLOGICAL PROPERTIES OF RICKETTSIA-TSUTSUGAMUSHI, KAWASAKI STRAIN, ISOLATED FROM A PATIENT IN KYUSHU
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DOI：
10.1111/j.1348-0421.1986.tb02988.x
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发表时间：
1986-01-01
影响因子：
2.6
通讯作者：
KAWAMURA, A
中科院分区：
文献类型：
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作者：
YAMAMOTO, S;KAWABATA, N;KAWAMURA, A
从日本九州宫崎县恙虫病（恙虫病）患者中分离到9株恙虫病立克次体。通过间接免疫荧光、间接免疫过氧化物酶或免疫印迹技术对这些患者的血清和分离株进行免疫学分析。在患者血清与原型菌株Gilliam、Karp和Kato以及分离株的反应分析中，包括川崎在内的两名患者的血清与从相应患者中回收的两种分离株显示出相似的特征和交叉反应，但与原型菌株的反应较弱。用豚鼠抗分离株和原型株的多克隆抗体，川崎株与Gilliam株显示出一定程度的交叉反应，但与Karp株或Kato株均无交叉反应，与Shimokoshi株也无交叉反应，已知Shimokoshi株与原型株的抗原性不同。此外，针对Gilliam、Karp和Kato菌株的菌株特异性鼠单克隆抗体与川崎菌株完全不反应。这些结果表明，川崎株可能与原型株和Shimokoshi株的抗原性不同。仅在9个分离株中发现两个相同抗原型的菌株（川崎），表明宫崎县存在川崎型立克次体。Shimokoshi株也不与这些株特异性单克隆抗体发生反应，表明R.抗原性不同于原型株如川崎和Shimokoshi株的恙虫病可通过它们与这些单克隆抗体的非反应性而容易地识别。
Nine isolates of Rickettsia tsutsugamushi were obtained from patients with Tsutsugamushi disease (scrub typhus) in Miyazaki Prefecture in Kyushu. Immunological analyses of these patients'' sera and the isolates were performed by indirect immunofluorescence, indirect immunoperoxidase or immunoblotting techniques. In the analysis of reactions of the patients'' sera with the prototype strains Gilliam, Karp, and Kato and with the isolates, sera from two patients, including Kawasaki, showed similar profiles and cross-reaction with the two isolates recovered from the corresponding patients, but reacted only weakly with the prototype strains. With guinea pig polyclonal antibodies against the isolate and prototype strains, Kawasaki strain showed some degree of cross-reaction with Gilliam strain but not with either Karp or Kato strain, nor with Shimokoshi strain which is known to be different antigenically from the prototype strains. Additionally, strain-specific murine monoclonal antibodies against Gilliam, Karp, and Kato strains did not react at all with Kawasaki strain. These results suggest that the Kawasaki strain may be different antigenically from the prototype strains and Shimokoshi strain. The finding two strains of the same antigenic type (Kawasaki) among only nine isolates suggests the presence of Kawasaki-type rickettsiae in Miyazaki Prefecture. Shimokoshi strain also did not react with these strain-specific monoclonal antibodies, suggesting that strains of R. tsutsugamushi antigenically distinct from the prototype strains, such as Kawasaki and Shimokoshi strains, may easily be recognized by their non-reactivity with these monoclonal antibodies.