Identification in transgenic animals of the Drosophila decapentaplegic sequences required for embryonic dorsal pattern formation.
Identification in transgenic animals of the Drosophila decapentaplegic sequences required for embryonic dorsal pattern formation.
复制标题
在转基因动物中鉴定胚胎背侧模式形成所需的果蝇十肢瘫痪序列。
DOI：
10.1101/gad.1.6.615
复制
发表时间：
1987
影响因子：
10.5
通讯作者：
Goodman,W
中科院分区：
文献类型：
--
作者：
Hoffmann,FM;Goodman,W
果蝇十足截瘫（dpp）基因的突变等位基因影响胚胎背腹模式的形成，幼虫的生存能力和成虫角质层的形成。这个功能阵列所需的dpp DNA跨度近50 kb。我们报道了dpp基因的胚胎致死性、腹化等位基因在转基因动物中被野生型dpp DNA的8 kb片段所拯救。在两种情况下，从胚胎致死性到成虫生存能力都可以完全恢复：一种是单倍体致死性dpp基因半合子的动物，另一种是两个隐性致死性胚胎等位基因中的任何一个半合子的动物。在没有dpp的胚胎中，背角质层向腹角质层的转化被dpp转座子的一个拷贝所阻断；两个副本允许幼虫孵化。足以实现这些胚胎功能的dpp部分编码一种与转化生长因子- β (tgf - β)蛋白家族同源的蛋白（Padgett et al. 1987）。dpp的幼虫和想象磁盘功能不是由8 kb的基因部分拯救的，必须从50 kb的DNA中获得额外的序列。
Mutant alleles of the Drosophila decapentaplegic (dpp) gene affect embryonic dorsal-ventral pattern formation, larval viability, and adult cuticle formation from the imaginal disks. The dpp DNA required for this array of functions spans almost 50 kb. We report that the embryonic lethal, ventralizing alleles of the dpp gene are rescued in transgenic animals by an 8-kb fragment of the wild-type dpp DNA. Full rescue, from embryonic lethality to adult viability, is obtained in two situations: in animals hemizygous for the haplolethal dpp gene, and in animals hemizygous for either of two recessive embryonic lethal alleles. In embryos null for dpp, the transformation of dorsal cuticle to ventral cuticle is blocked by one copy of the dpp transposon; two copies permit the hatching of the larvae. The portion of dpp sufficient for these embryonic functions encodes a protein with homology to the transforming growth factor-beta (TGF-beta) family of proteins (Padgett et al. 1987). The larval and imaginal disk functions of dpp are not rescued by the 8-kb portion of the gene and must require additional sequences from the 50 kb of DNA.