A Bacillus sp. strain with antagonistic activity against Fusarium graminearum kills Microcystis aeruginosa selectively.
A Bacillus sp. strain with antagonistic activity against Fusarium graminearum kills Microcystis aeruginosa selectively.
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DOI：
10.1016/j.scitotenv.2017.01.055
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发表时间：
2017-04
期刊：
影响因子：
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通讯作者：
H. Xuan;Xian-zhu Dai;Jing Li;Xiaohui Zhang;Caiyun Yang;Feng Luo
中科院分区：
文献类型：
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作者：
H. Xuan;Xian-zhu Dai;Jing Li;Xiaohui Zhang;Caiyun Yang;Feng Luo
蓝藻水华危害严重，造成严重的环境问题和经济损失，严重威胁人类健康。在本研究中，一株芽孢杆菌(Bacillussp.)从紫色土中分离到一株对植物病原真菌禾谷镰刀菌有较强拮抗活性的菌株，命名为AF-1。AF-1在低细胞密度(1.6×103cfu/mL)下选择性杀灭铜绿微囊藻，对微囊藻显示出最强的杀菌活性。铜绿假单胞菌-843(Ae=0.93%，t=6.6d)。菌株AF-1产生的杀藻物质在35~100℃温度范围内稳定，pH在3~11范围内稳定。AF-1无细胞培养滤液导致胞内ROS过量积累、细胞死亡和胞内成分外流。铜绿假单胞菌-843细胞。AF-1无细胞培养滤液对NIES 843细胞DNA修复、光合作用和微囊藻毒素合成等基因的表达有显著影响。AF-1有可能被开发为一种控制氰化细菌和赤霉病的双功能生防剂。由谷物引起的植物病害。
Cyanobacterial harmful algal blooms (CyanoHABs) cause severe environmental problems, economic losses and threaten human health seriously. In the present study, aBacillussp. strain, designated as AF-1, with strong antagonistic activity against plant pathogenic fungusFusarium graminearumwas isolated from purple soil.Bacillussp. AF-1 selectively killedMicrocystisaeruginosaat low cell density (1.6 × 103cfu/mL), and showed the strongest bactericidal activity againstM. aeruginosaNIES-843 (Ae= 93%,t= 6 d). The algicidal substances originated from strain AF-1 were stable in the temperature range of 35–100 °C, and pH range of 3–11. Cell-free filtrate of AF-1 culture caused excessive accumulation of intracellular reactive oxygen species (ROS), cell death and the efflux of intracellular components ofM. aeruginosaNIES-843 cells. The expression of genesrecA,psbA1,psbD1,rbcLandmcyB, involved in DNA repair, photosynthesis and microcystin synthesis of NIES 843, were significantly influenced by the cell-free filtrate of AF-1 culture.Bacillussp. AF-1 has the potential to be developed as a bifunctional biocontrol agent to control CyanoHABs andF. graminearumcaused plant disease.