Protein film electrochemistry
Protein film electrochemistry
复制标题
DOI：
10.1038/s43586-023-00262-7
复制
发表时间：
2023-10-19
期刊：
影响因子：
--
通讯作者：
Sutton-Cook，Alexander L.
中科院分区：
文献类型：
--
作者：
Butt，Julea N.;Jeuken，Lars J. C.;Sutton-Cook，Alexander L.
蛋白质膜电化学（PFE）是一种用于研究氧化还原蛋白质性质的强有力的电分析技术。所研究的蛋白质作为（亚）单层膜吸附在电极表面上。固定化蛋白质和工作电极之间的直接电子转移产生电流，该电流可视化并定量蛋白质内发生的氧化还原过程。{1} PFE {2}的优点包括样品要求低{3}-{4}通常小于纳摩尔蛋白质{5}-{6}高灵敏度{7}，{8}以及在电化学电位和时间域中分辨氧化还原化学的能力。本入门提供了使用PFE进行半反应（氧化还原反应）和耦合化学过程（包括配体结合、配体解结合和氧化还原催化）的定量热力学和动力学描述的指南。重点介绍了PFE在生物传感器、能量转换和酶解等方面的应用。最后，国家的艺术和新的实验和理论方法的前景进行了讨论。
Protein film electrochemistry (PFE) is a powerful suite of electroanalytical techniques used to investigate the properties of redox proteins. The proteins under investigation are adsorbed as a (sub-)monolayer film on an electrode surface. Direct electron transfer between the immobilized protein and working electrode gives rise to an electrical current that visualizes and quantifies redox processes occurring within the protein. Advantages of PFE include low sample requirements — typically less than a nanomole protein — high sensitivity and the ability to resolve redox chemistry in the electrochemical potential and time domains. This Primer provides a guide to using PFE for quantitative thermodynamic and kinetic descriptions of half-reactions (redox reactions) and coupled chemical processes, including ligand binding, ligand unbinding and redox catalysis. Applications of PFE in developing biosensors, facilitating energy conversion and resolving enzyme mechanisms are highlighted. Finally, the state of the art and prospects for novel experimental and theoretical approaches are discussed.