Measurement of steroid hormones in saliva: Effects of sample storage condition
Measurement of steroid hormones in saliva: Effects of sample storage condition
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唾液中类固醇激素的测量：样品储存条件的影响
DOI：
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发表时间：
2013
影响因子：
2.1
通讯作者：
K. Stokes
中科院分区：
文献类型：
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作者：
R. Toone;O. Peacock;Alan A. Smith;D. Thompson;S. Drawer;C. Cook;K. Stokes
唾液中类固醇激素的测量在精英运动环境中越来越普遍。然而，这种环境可能会强制处理和储存实践，从而在激素浓度的测量中引入误差。我们评估了储存温度和持续时间对唾液类固醇水平重现性的影响。9名健康成年人在两个不同的场合提供了上午和下午的唾液样本。将每份样本分成相同的唾液等分试样，在− 80°C或− 20°C下长期（即28和84天）储存（测试第1天），在4°C或20°C下短期（即1、3、7和14天）储存（测试第2天）。使用ELISA分析样品的皮质醇、睾酮和雌二醇。在非冷冻条件下，从基线到7天，睾酮（-26 ± 15%）和雌二醇（-58 ± 17%）下降，但皮质醇浓度没有下降（p < 0.001）。在室温下储存的样品中，该下降幅度大于在冰箱中储存的样品（p ≤ 0.01）。在冷冻条件下储存28和/或84天后，所有激素的测量浓度均出现微小但显著的变化（p ≤ 0.01），但这些变化通常在基线浓度的12%范围内，可能部分由试验间变异性解释。用于分析皮质醇、睾酮和雌二醇的全唾液样本应在采集后24小时内在− 20°C或以下冷冻，并在28天内进行分析。在高于− 20°C的温度下储存用于测量睾酮和雌二醇的样品可能会对测量浓度产生较大的误差方差。
Abstract Measurement of steroid hormones in saliva is increasingly common in elite sport settings. However, this environment may enforce handling and storage practices that introduce error in measurement of hormone concentrations. We assessed the influence of storage temperature and duration on reproducibility of salivary steroid levels. Nine healthy adults provided morning and afternoon saliva samples on two separate occasions. Each sample was divided into identical saliva aliquots which were stored long-term (i.e. 28 and 84 days) at − 80°C or − 20°C (testing day 1), and short-term (i.e. 1, 3, 7 and 14 days) at 4°C or 20°C (testing day 2). Samples were analyzed for cortisol, testosterone and estradiol using ELISA. In non-freezer conditions, there was a decrease from baseline to 7 days in testosterone (− 26 ± 15%) and estradiol (− 58 ± 17%) but not cortisol concentrations (p < 0.001). This decrease was larger in samples stored at room temperature than in the refrigerator (p ≤ 0.01). There were small but significant changes in measured concentrations of all hormones after 28 and/or 84 days of storage in freezer conditions (p ≤ 0.01), but these were generally within 12% of baseline concentrations, and may be partly explained by inter-assay variability. Whole saliva samples to be analyzed for cortisol, testosterone and estradiol should be frozen at − 20°C or below within 24 h of collection, and analyzed within 28 days. Storage of samples for measurement of testosterone and estradiol at temperatures above − 20°C can introduce large error variance to measured concentrations.
影响因子：
9.3
作者：
G. Schwartz;S. Turner
通讯作者：
G. Schwartz;S. Turner