YgiW homologous gene from Pseudomonas aeruginosa 25W is responsible for tributyltin resistance.
YgiW homologous gene from Pseudomonas aeruginosa 25W is responsible for tributyltin resistance.
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DOI：
10.2323/jgam.58.283
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
K. Fukushima;Santosh Kumar;S. Suzuki
中科院分区：
文献类型：
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作者：
K. Fukushima;Santosh Kumar;S. Suzuki
从耐三丁基锡（TBT）海洋细菌铜绿假单胞菌25W中分离到一个TBT抗性基因。该基因与保存在铜绿假单胞菌PAO1数据库（http：//www.example.com）中的PA0320相同。www.pseudomonas.com推测的PA0320的氨基酸序列似乎与大肠杆菌和肠道沙门氏菌的YgiW蛋白同源。PA0320的缺失突变体在TBT存在下表现出生长速率的降低。镉，汞，过氧化氢和酸性pH值的缺失突变体的敏感性试验表明，增加的敏感性。PA0320在植物抗TBT胁迫和产生活性氧方面具有一定的作用。
A tributyltin (TBT) resistance gene was isolated from the TBT-resistant marine origin bacterium Pseudomonas aeruginosa 25W. This gene was identical to PA0320 deposited in the P. aeruginosa PAO1 database (http://www.pseudomonas.com). The deduced amino acid sequence of PA0320 appears to be homologous to the YgiW proteins of Escherichia coli and Salmonella enterica. The deletion mutant of PA0320 showed a reduction of growth rate in the presence of TBT. A susceptibility test to cadmium, mercury, hydrogen peroxide and acidic pH in the deletion mutant showed an increasing susceptibility to them. PA0320 plays a certain role in stress tolerance against TBT as well as in stressors producing reactive oxygen species.