EFFECTS OF PRODUCTS OF ACTIVATED LEUKOCYTES (LYMPHOKINES AND MONOKINES) ON THE GROWTH OF MALIGNANT TROPHOBLAST CELLS-INVITRO
EFFECTS OF PRODUCTS OF ACTIVATED LEUKOCYTES (LYMPHOKINES AND MONOKINES) ON THE GROWTH OF MALIGNANT TROPHOBLAST CELLS-INVITRO
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DOI：
10.1016/0002-9378(88)90810-1
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发表时间：
1988-01-01
影响因子：
9.8
通讯作者：
ANDERSON, DJ
中科院分区：
文献类型：
--
作者：
BERKOWITZ, RS;HILL, JA;ANDERSON, DJ
将来自活化的白细胞培养物的上清液以及单个淋巴因子和单核因子加入体外培养的JEG-3人妊娠绒毛膜癌细胞中，并测量对细胞增殖的影响。活化的白细胞培养上清液，重组γ-干扰素、肿瘤坏死因子和集落刺激因子显著抑制JEG-3增殖。相反，高剂量的白细胞介素1和2刺激JEG-3增殖。低剂量的B细胞生长因子刺激JEG-3增殖，而最高剂量的抑制。进一步了解淋巴因子和单核因子对滋养细胞生长的影响，可能会对影响妊娠滋养细胞肿瘤的早期妊娠发展和肿瘤-宿主相互作用的免疫机制提供重要的见解。
Supernatants from activated leukocyte cultures and individual lymphokines and monokines were added to cultures of JEG-3 human gestational choriocarcinoma cells in vitro, and effects on cell proliferation were measured. Activated leukocyte culture supernatants, recombinant .gamma.-interferon, tumor necrosis factor, and colony-stimulating factor significantly inhibited JEG-3 proliferation. In contrast, high doses of both interleukin 1 and 2 stimulated JEG-3 proliferation. Low doses of B cell growth factor stimulated JEG-3 proliferation, whereas the highest dose was inhibitory. Further understanding of the effects of lymphokines and monokines on trophoblastic growth may provide important insights into immunologic mechanisms affecting early pregnancy development and tumor-host interactions in gestational trophoblastic neoplasia.