Oral administration of viable Bifidobacterium pseudolongum strain Patronus modified colonic microbiota and increased mucus layer thickness in rat
Oral administration of viable Bifidobacterium pseudolongum strain Patronus modified colonic microbiota and increased mucus layer thickness in rat
复制标题
DOI：
10.1093/femsec/fiy177
复制
发表时间：
2018-11-01
影响因子：
4.2
通讯作者：
Pochart, Philippe
中科院分区：
文献类型：
--
作者：
Mangin, Irene;Dossou-Yovo, Flore;Pochart, Philippe
本研究旨在评估大鼠口服活双歧杆菌引起的结肠微生物群的改变和粘液层厚度的变化。对用假长双歧杆菌（Bifidobacterium pseudolongum）菌株Patronus（每天10（10）个细菌，持续7天）喂养的大鼠进行研究。这种细菌给药导致粘液厚度大幅增加（57%，P < 0.05）。细菌16S rRNA基因的定量PCR和高通量测序均显示，在喂食Patronus菌株的大鼠的微生物群中，双歧杆菌属的量显著增加，与嗜粘蛋白阿克曼氏菌的减少相关。粘液厚度的增加可能是由于双歧杆菌本身的增加或通过A. muciniphila，一种主要的粘蛋白降解物种。由于粘液层在肠道保护中发挥着至关重要的作用，我们的数据揭示了研究粘液降解细菌对于了解肠道疾病等疾病的潜在病因和实施新的治疗策略的重要性。
This study aimed at evaluating the alteration of the colonic microbiota and the changes in the mucus layer thickness induced by oral administration of living bifidobacteria in rats. The study was performed on rats fed with Bifidobacterium pseudolongum strain Patronus (10(10) bacteria per day for 7 days). This bacterial administration led to a large increase of mucus thickness (57%, P < 0.05). Both quantitative PCR and high-hroughput sequencing of bacterial 16S rRNA gene revealed a significant increase of the amount of the Bifidobacterium genus in the microbiota of rats fed with the strain Patronus, associated with a decrease of Akkermansia muciniphila. The increase in mucus thickness could be due to an increase of the bifidobacteria per se or via the decrease of A. muciniphila, a major mucin-degrading species. As the mucus layer plays an essential role in gut protection, our data enlighten the importance of studying mucus-degrading bacteria for understanding the underlying etiology of diseases such as intestinal bowel diseases and to implement new therapeutic strategies.