Expression of the beta 6 integrin subunit in development, neoplasia and tissue repair suggests a role in epithelial remodeling.
Expression of the beta 6 integrin subunit in development, neoplasia and tissue repair suggests a role in epithelial remodeling.
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发表时间：
1995-06
影响因子：
4
通讯作者：
J. Breuss;J. Gallo;H. DeLisser;I. Klimanskaya;H. Folkesson;J. Pittet;S. Nishimura;K. Aldape;
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文献类型：
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作者：
J. Breuss;J. Gallo;H. DeLisser;I. Klimanskaya;H. Folkesson;J. Pittet;S. Nishimura;K. Aldape;
在培养的上皮细胞中鉴定出α v β 6整联蛋白，其功能为纤连蛋白受体。我们现在已经使用单克隆抗体来确定β 6亚基在正常和病理性人类或灵长类动物组织中的体内表达模式，以及在实验性伤口愈合或诱导的肺损伤期间的表达模式。结果表明，β 6的表达仅限于上皮细胞，并与形态发生事件，肿瘤发生和上皮修复平行上调。在肾脏、肺和皮肤的发育过程中，我们发现β 6由特定类型的上皮细胞表达，而在正常成人肾脏、肺和皮肤中几乎检测不到。相反，我们在几种类型的癌中检测到高水平表达。例如，β 6几乎总是在来源于口腔粘膜的鳞状细胞癌中新表达，通常局灶性地位于肿瘤岛的浸润边缘。在实验性表皮伤口愈合过程中，在伤口边缘的迁移角质形成细胞中β 6的表达也上调。类似地，在由注射活细菌引起的肺损伤期间，在II型肺泡上皮细胞中诱导β 6表达。我们还观察到β 6在成人肺和肾的亚临床炎症病灶部位的表达，以及肺或肾慢性或急性炎症患者的各种临床标本中的表达。从这些发现和早期的结果，我们假设α v β 6影响细胞的扩散，迁移和生长过程中的上皮细胞的重组，在发展，组织修复和肿瘤。
The alpha v beta 6 integrin was identified in cultured epithelial cells and functions as a fibronectin receptor. We have now used monoclonal antibodies to determine in vivo expression patterns of the beta 6 subunit in normal and pathological human or primate tissues, and during experimental wound healing or induced lung injury. The results indicate that beta 6 expression is restricted to epithelia and is up-regulated in parallel with morphogenetic events, tumorigenesis, and epithelial repair. During development of the kidney, lung, and skin, we found that beta 6 is expressed by specific types of epithelial cells, whereas it is mostly undetectable in normal adult kidney, lung and skin. In contrast, we detected high-level expression in several types of carcinoma. For example, beta 6 is almost invariably neo-expressed in squamous cell carcinomas derived from the oral mucosa, often focally localized at the infiltrating edges of tumor islands. Expression of beta 6 is also upregulated in migrating keratinocytes at the wound edge during experimental epidermal wound healing. Similarly, beta 6 expression is induced in type II alveolar epithelial cells during lung injury caused by injection of live bacteria. We also observed beta 6 expression in adult lungs and kidneys at focal sites of subclinical inflammation, as well as in a variety of clinical specimens from patients with chronic or acute inflammation of the lungs or kidneys. From these findings and earlier results, we hypothesize that alpha v beta 6 affects cell spreading, migration and growth during reorganization of epithelia in development, tissue repair, and neoplasia.