Sequencing of peptoid peptidomimetics by Edman degradation
Sequencing of peptoid peptidomimetics by Edman degradation
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DOI：
10.1016/s0040-4039(98)00556-5
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发表时间：
1998-05-21
影响因子：
1.8
通讯作者：
Liskamp, RMJ
中科院分区：
文献类型：
--
作者：
Boeijen, A;Liskamp, RMJ
描述了通过测序直接鉴定树脂结合的类肽模拟物。用异硫氰酸苯酯处理类肽的N-末端，之后将N-末端类肽残基从树脂上裂解为其硫代乙内酰脲衍生物。为了推导类肽序列，将所得硫代乙内酰脲的HPLC保留时间与独立制备的参比硫代乙内酰脲的HPLC保留时间进行比较。(C)1998爱思唯尔科技有限公司版权所有。
The direct identification of resin-bound peptoid peptidomimetics by sequencing is described. The N-terminus of the peptoid was treated with phenyl isothiocyanate, after which the N-terminal peptoid residue was cleaved from the resin as its thiohydantoin derivative. For deduction of the peptoid sequence, the HPLC retention times of the obtained thiohydantoins were compared to those of independently prepared reference thiohydantoins. (C) 1998 Elsevier Science Ltd. All rights reserved.