Impact of knee joint loading on fragmentation of serum cartilage oligomeric matrix protein
Impact of knee joint loading on fragmentation of serum cartilage oligomeric matrix protein
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DOI：
10.1002/jor.24586
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发表时间：
2020-01-15
影响因子：
2.8
通讯作者：
Niehoff, Anja
中科院分区：
文献类型：
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作者：
Firner, Sara;Zaucke, Frank;Niehoff, Anja
本研究旨在探讨膝关节机械负荷对血清软骨寡聚基质蛋白（COMP）破碎模式的影响。10名健康男性使用被动或主动膝关节矫形器（+30.9 N中心点m外屈力矩）在跑步机上跑步（30分钟；v = 2.2 m/s）。分析下肢力学、血清COMP水平和碎片模式（基线；跑步后0、0.5、1、2小时）。使用主动矫形器跑步可提高膝关节屈曲力矩、踝关节背屈度和膝关节屈曲角度（P < 0.05）。有提高血清COMP(+ 25%,前:8.9 + / - 2.4 U / l;: 10.7 + / - 1.9 U / l, P =措施),COMP五聚物/四聚物(+ 88%;1.88 + / - 0.81,P = .007)、三聚物(+ 209%;3.09 + / - 2.65,P = .005),和单体(+ 78%;1.78 + / - 0.85,P = .007)与被动矫形器和运行后血清COMP(+ 41%,前:8.5 + / - 2.7 U / l;: 11.3 + / - 2.1 U / l P <措施),COMP五聚物/四聚物(+ 57%;1.57 + / - 0.39,P = .007)、三聚物(+ 86%;1.86 + / - 0.47,P = .005),和单体(+ 19%;1.19 +/- 0.34, P = 0.114)。碎片增加可能表明跑步时软骨中COMP释放。有趣的是,0.5 h 2小时与被动矫形器运行后,三聚物(0.5小时:2.73 + / - 3.40,P = .029; 2小时:2.33 + / - 2.88,P = .037),和单体(0.5小时:2.23 + / - 2.33,P = .007; 1小时:2.55 + / - 1.96,P = .012; 2小时:2.65 + / - 2.50,P = .009)在运行时增加和活跃的矫形器,五聚物/四聚物(1小时:0.79 + / - 0.28,P = .029),和三聚物(1小时:0.63 + / - 0.14,P = .005; 2小时:0.68 + / - 0.34,P = .047)下降。COMP的退化和间隙似乎根据关节的载荷特性而变化。
The aim of the study was to examine the effect of mechanical knee joint loading on the fragmentation pattern of serum cartilage oligomeric matrix protein (COMP). Ten healthy men ran with knee orthoses that were passive or active (+30.9 N center dot m external flexion moments) on a treadmill (30 minute; v = 2.2 m/s). Lower-limb mechanics, serum COMP levels, and fragmentation patterns (baseline; 0, 0.5, 1, 2 hours postrunning) were analyzed. Running with active orthoses enhanced knee flexion moments, ankle dorsiflexion, and knee flexion angles (P < .05). There was an increase in serum COMP (+25%; pre: 8.9 +/- 2.4 U/l; post: 10.7 +/- 1.9 U/l, P = .001), COMP pentamer/tetramer (+88%; 1.88 +/- 0.81, P = .007), trimer (+209%; 3.09 +/- 2.65, P = .005), and monomer (+78%; 1.78 +/- 0.85, P = .007) after running with passive orthoses and in serum COMP (+41%; pre: 8.5 +/- 2.7 U/l; post: 11.3 +/- 2.1 U/l, P < .001), COMP pentamer/tetramer (+57%; 1.57 +/- 0.39, P = .007), trimer (+86%; 1.86 +/- 0.47, P = .005), and monomer (+19%; 1.19 +/- 0.34, P = .114) after running with active orthoses. Increased fragmentation might indicate COMP release from cartilage while running. Interestingly, 0.5 h up to 2 hours after running with passive orthoses, trimer (0.5 hour: 2.73 +/- 3.40, P = .029; 2 hours: 2.33 +/- 2.88, P = .037), and monomer (0.5 hour: 2.23 +/- 2.33, P = .007; 1 hour: 2.55 +/- 1.96, P = .012; 2 hours: 2.65 +/- 2.50, P = .009) increased while after running with active orthoses, pentamer/tetramer (1 hour: 0.79 +/- 0.28, P = .029), and trimer (1 hour: 0.63 +/- 0.14, P = .005; 2 hours: 0.68 +/- 0.34, P = .047) decreased. It seems that COMP degradation and clearance vary depending on joint loading characteristics.