Dentin Biomodification Potential Depends on Polyphenol Source
Dentin Biomodification Potential Depends on Polyphenol Source
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DOI：
10.1177/0022034514523783
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发表时间：
2014-04-01
影响因子：
7.6
通讯作者：
Bedran-Russo, A. K.
中科院分区：
文献类型：
--
作者：
Aguiar, T. R.;Vidal, C. M. P.;Bedran-Russo, A. K.
虽然原花青素（PAC）修饰牙本质，不同的PAC来源的有效性和与其特定的化学成分的相关性仍然是未知的。本研究描述了7种植物的富含PAC的天然提取物的化学分析，使用超高压/高效液相色谱法（UHPLC）来确定这些提取物的总体组成，并同时全面评估其对牙本质性质的影响。使用Folin-Ciocalteau测定法测定提取物的总多酚含量（作为没食子酸当量）。牙本质生物改性通过弹性模量、质量变化和对酶降解的抵抗力来评估。从葡萄属葡萄、可可、茶树和马尾松中提取的具有高多酚和PAC含量的提取物引起弹性模量和质量的显著增加。超高效液相色谱分析表明存在多种类型的多酚，从简单的酚酸到低聚PAC和高度缩合的单宁。所有实验组均观察到对酶降解的保护作用;然而，在植物提取物之间观察到统计学显著差异。研究结果提供了明确的证据表明，牙本质生物活性的PAC是源依赖性的，从复杂的PAC混合物的浓度和特定的化学成分的组合。
Although proanthocyanidins (PACs) modify dentin, the effectiveness of different PAC sources and the correlation with their specific chemical composition are still unknown. This study describes the chemical profiling of natural PAC-rich extracts from 7 plants using ultra high pressure/performance liquid chromatography (UHPLC) to determine the overall composition of these extracts and, in parallel, comprehensively evaluate their effect on dentin properties. The total polyphenol content of the extracts was determined (as gallic acid equivalents) using Folin-Ciocalteau assays. Dentin biomodification was assessed by the modulus of elasticity, mass change, and resistance to enzymatic biodegradation. Extracts with a high polyphenol and PAC content from Vitis vinifera, Theobroma cacao, Camellia sinensis, and Pinus massoniana induced a significant increase in modulus of elasticity and mass. The UHPLC analysis showed the presence of multiple types of polyphenols, ranging from simple phenolic acids to oligomeric PACs and highly condensed tannins. Protective effect against enzymatic degradation was observed for all experimental groups; however, statistically significant differences were observed between plant extracts. The findings provide clear evidence that the dentin bioactivities of PACs are source dependent, resulting from a combination of concentration and specific chemical constitution of the complex PAC mixtures.