Increase in Ascorbate Content of Transgenic Tobacco Plants Overexpressing the Acerola (Malpighia glabra) Phosphomannomutase Gene
Increase in Ascorbate Content of Transgenic Tobacco Plants Overexpressing the Acerola (Malpighia glabra) Phosphomannomutase Gene
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DOI：
10.1093/pcp/pcn206
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发表时间：
2009-02-01
影响因子：
4.9
通讯作者：
Esaka, Muneharu
中科院分区：
文献类型：
--
作者：
Badejo, Adebanjo A.;Eltelib, Hani A.;Esaka, Muneharu
磷酸甘露变位酶（PMM; EC 5.4.2.8）在用于l-抗坏血酸（阿萨）的生物合成的SmirnoffWheeler途径中催化甘露糖-6-磷酸向甘露糖-1-磷酸的相互转化。我们从针叶树（Malpighia glabra）中克隆了PMM cDNA，针叶树是一种含有大量阿萨A的植物。成熟果实和叶片的阿萨含量与PMM基因的表达相关。针叶、番茄和拟南芥叶片中PMM活性与各自的阿萨含量相关。过表达金虎尾PMM基因的转基因烟草植株中阿萨含量比野生型增加了约2倍，且与PMM转录水平和活性相关。
Phosphomannomutase (PMM; EC 5.4.2.8) catalyzes the interconversion of mannose-6-phosphate to mannose-1-phosphate in the SmirnoffWheeler pathway for the biosynthesis of l-ascorbic acid (AsA). We have cloned the PMM cDNA from acerola (Malpighia glabra), a plant containing an enormous amount of AsA. The AsA contents correlate with the PMM gene expression of the ripening fruits and leaves. The PMM activities in the leaves of acerola, tomato and Arabidopsis correlate with their respective AsA contents. Transgenic tobacco plants overexpressing the acerola PMM gene showed about a 2-fold increase in AsA contents compared with the wild type, with a corresponding correlation with the PMM transcript levels and activities.