Heterogeneity of liver-kidney microsomal autoantibodies in chronic hepatitis C and D virus infection.
Heterogeneity of liver-kidney microsomal autoantibodies in chronic hepatitis C and D virus infection.
复制标题
慢性丙型肝炎和丁型肝炎病毒感染中肝肾微粒体自身抗体的异质性。
DOI：
10.1016/0016-5085(95)90074-8
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发表时间：
1995
期刊：
影响因子：
29.4
通讯作者：
M. Manns
中科院分区：
文献类型：
--
作者：
M. Durazzo;T. Philipp;F. V. van Pelt;B. Lüttig;E. Borghesio;G. Michel;E. Schmidt;S. Loges;M. Rizzetto;M. Manns
抗肝肾微粒体（LKM）自身抗体发生在慢性丙型肝炎和丁型肝炎感染的患者中。由于不同的免疫荧光模式，丙型肝炎和丁型肝炎中的抗体分别被称为LKM-1和LKM-3。本研究的目的是评估LKM-1和LKM-3抗体的不同特异性。方法采用免疫荧光法、酶联免疫吸附试验、结果34%的LKM-1血清可与50 kD的细胞色素P4502 D 6反应。此外，一部分血清识别59或70千道尔顿抗原，45%的血清在Western印迹中不反应。最近，主要的LKM-3抗原被确定为尿苷二磷酸-葡萄糖醛酸转移酶（UGT）上表达的自身表位。7份LKM-3阳性血清与重组兔家族1 UGT反应。抗LKM-1阳性的丙型肝炎血清均不与UGT反应。在酶联免疫吸附试验中，对肝微粒体蛋白的抗体反应结束时，抗原预处理与十二烷基硫酸钠，确认抗体识别构象epitopes.ConclusionsLKM-1抗体在丙型肝炎更异质性和不同的抗原反应相比，LKM-3抗体在丁型肝炎。
Background/AimsAnti-liver-kidney microsomal (LKM) autoantibodies occur in a proportion of patients with chronic hepatitis C and D infections. Because of different immunofluorescence patterns, antibodies in hepatitis C and D were termed LKM-1 and LKM-3, respectively. The aim of the present study was to evaluate the different specificities of LKM-1 and LKM-3 antibodies.MethodsForty-nine samples of LKM-1 sera and 16 samples of LKM-3 sera were studied for reactivity against rat and human liver microsomal proteins by immunofluorescence, enzyme-linked immunosorbent assay, and Western blot.ResultsThirty-four percent of the LKM-1 sera reacted with 50-kilodalton cytochrome P4502D6 in Western blot. In addition, a proportion of the sera recognized either a 59- or 70-kilodalton antigen, and 45% of the sera did not react in Western blot. Recently, the major LKM-3 antigen was identified as an autoepitope expressed on uridine diphosphate-glucuronosyltransferases (UGT). Seven LKM-3-positive sera reacted with recombinant rabbit family one UGT. None of the anti-LKM-1-positive hepatitis C sera reacted with UGT. Antibody reactivity against liver microsomal proteins in enzyme-linked immunosorbent assay ended when antigens were pretreated with sodium dodecyl sulfate, confirming that antibodies recognize conformational epitopes.ConclusionsLKM-1 antibodies in hepatitis C are more heterogeneous and react with different antigens compared with LKM-3 antibodies in hepatitis D.
影响因子：
15.9
作者：
MANNS, MP;GRIFFIN, KJ;JOHNSON, EF
通讯作者：
JOHNSON, EF
细胞色素 P450IA2 鉴定为人类自身抗原。
影响因子：
3.9
作者：
Manns,MP;Griffin,KJ;Quattrochi,LC;Sacher,M;Thaler,H;Tukey,RH;Johnson,EF
通讯作者：
Johnson,EF
慢性活动性肝炎中的肝/肾微粒体1抗体可识别特定形式的肝细胞色素 P-450。
影响因子：
29.4
作者：
Waxman,DJ;Lapenson,DP;Krishnan,M;Bernard,O;Kreibich,G;Alvarez,F
通讯作者：
Alvarez,F