Lipid composition of cohesive and desquamated corneocytes from mouse ear skin.
Lipid composition of cohesive and desquamated corneocytes from mouse ear skin.
复制标题
小鼠耳部皮肤粘性和脱落角质细胞的脂质成分。
DOI：
10.1111/1523-1747.ep12284246
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Mackenzie,IC
中科院分区：
文献类型：
--
作者：
Ranasinghe,AW;Wertz,PW;Downing,DT;Mackenzie,IC
器官培养系统已经被用来检测来自粘连和剥离的小鼠耳角质层材料的脂质成分的差异。在这种培养系统中，皮肤外植体显示的细胞复制和分化速度与体内观察的细胞复制和分化速度相当，长达2周，在此期间，松散或脱落的物质聚集在表面。测定了来自培养皮肤的完整角质层和松弛角质层以及新鲜制备的角质层的脂质成分。在所有3个病例中，非极性脂和神经酰胺的轮廓基本相同；一些非极性脂似乎起源于皮脂腺。在脱皮时检测到的唯一变化是胆固醇硫酸盐的减少和第二种类似极性的不明脂肪。在新鲜角质层或器官培养的角质层中，硫酸胆固醇占极脂的4-5%。在培养系统中积累的脱皮物质中，这一比例降低到0.4%。未鉴定的脂类从完整、新鲜或培养的角质层中极脂的1-2%下降到脱皮材料中的0.1%。讨论了硫酸胆固醇在角膜细胞凝聚力中的可能作用。
An organ culture system has been used to examine differences in the lipid compositions of materials derived from cohesive and desquamated mouse ear stratum corneum. Within this culture system, skin explants display rates of cell replication and differentiation comparable to those observed in vivo for up to 2 weeks and, during this period, loosened or dishesive material accumulates at the surface. Lipid compositions were determined for both intact and loosened stratum corneum derived from cultured skin and also for freshly prepared stratum corneum. In all 3 cases, the profiles of the nonpolar lipids and ceramides were essentially the same; some of the nonpolar lipids appeared to be of sebaceous origin. The only changes detected upon desquamation were reductions of cholesteryl sulfate and a second unidentified lipid of similar polarity. Cholesteryl sulfate constitutes 4–5% of the polar lipid in fresh stratum corneum or stratum corneum from organ culture. This is reduced to 0.4% in the desquamated material which accumulates in the culture system. The unidentified lipid decreases from 1–2% of the polar lipid in intact fresh or cultured stratum corneum to 0.1% in the desquamated material. The possible function of cholesteryl sulfate in corneocyte cohesion is discussed.