C57BL/6 and Swiss Webster Mice Display Differences in Mobility, Gliosis, Microcavity Formation and Lesion Volume After Severe Spinal Cord Injury.
C57BL/6 and Swiss Webster Mice Display Differences in Mobility, Gliosis, Microcavity Formation and Lesion Volume After Severe Spinal Cord Injury.
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DOI：
10.3389/fncel.2018.00173
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发表时间：
2018
影响因子：
5.3
通讯作者：
Perrin FE
中科院分区：
文献类型：
--
作者：
Noristani HN;They L;Perrin FE
脊髓损伤（SCI）是一种神经病理学疾病，会造成巨大的身体和情感痛苦以及不可逆的残疾，给我们的社会带来巨大的社会/经济负担。多种小鼠品系的可用性对于研究SCI后潜在的病理生理反应是重要的。尽管应变差异已被证明直接影响不完全SCI后的自发功能恢复，但其在脊髓完全损伤后的影响尚不清楚。为了研究小鼠应变对严重SCI后恢复的影响，我们首先在两种不同遗传背景的小鼠（即C57 BL/6和Swiss韦伯斯特）中进行了脊髓完全横断后长达6周的行为分析。使用免疫组化，我们分析了胶质细胞反应不仅在不同的时间点损伤后，而且在不同的距离病变震中。使用CatWalk™和旷场分析进行的行为评估显示，在脊髓完全横断后，与C57 BL/6小鼠相比，Swiss韦伯斯特小鼠的移动性（使用平均速度测量）和前肢总感觉反应差异增加。综合组织学评估显示，与C57 BL/6小鼠相比，Swiss韦伯斯特小鼠的小胶质细胞/巨噬细胞反应性升高，星形胶质细胞增生中度增加，这与脊髓横断后微腔形成减少和病变体积减少有关。因此，我们的研究结果表明，增加流动性与增强胶质细胞增生和更好的组织保护后，完全横断脊髓。
Spinal cord injuries (SCI) are neuropathologies causing enormous physical and emotional anguish as well as irreversibly disabilities with great socio/economic burdens to our society. The availability of multiple mouse strains is important for studying the underlying pathophysiological response after SCI. Although strain differences have been shown to directly affect spontaneous functional recovery following incomplete SCI, its influence after complete lesion of the spinal cord is unclear. To study the influence of mouse strain on recovery after severe SCI, we first carried out behavioral analyses up to 6 weeks following complete transection of the spinal cord in mice with two different genetic backgrounds namely, C57BL/6 and Swiss Webster. Using immunohistochemistry, we then analyzed glial cell reactivity not only at different time-points after injury but also at different distances from the lesion epicenter. Behavioral assessments using CatWalk™ and open field analyses revealed increased mobility (measured using average speed) and differential forelimb gross sensory response in Swiss Webster compared to C57BL/6 mice after complete transection of the spinal cord. Comprehensive histological assessment revealed elevated microglia/macrophage reactivity and a moderate increase in astrogliosis in Swiss Webster that was associated with reduced microcavity formation and reduced lesion volume after spinal cord transection compared to C57BL/6 mice. Our results thus suggest that increased mobility correlates with enhanced gliosis and better tissue protection after complete transection of the spinal cord.
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影响因子：
4.2
作者：
Desclaux, Mathieu;Perrin, Florence E.;Privat, Alain
通讯作者：
Privat, Alain
影响因子：
5.3
作者：
Brewer, KL;Bethea, JR;Yezierski, RP
通讯作者：
Yezierski, RP
星形胶质细胞疤痕形成有助于中枢神经系统轴突再生。
影响因子：
64.8
作者：
Anderson MA;Burda JE;Ren Y;Ao Y;O'Shea TM;Kawaguchi R;Coppola G;Khakh BS;Deming TJ;Sofroniew MV
通讯作者：
Sofroniew MV
影响因子：
3.7
作者：
Gerber YN;Sabourin JC;Rabano M;Vivanco Md;Perrin FE
通讯作者：
Perrin FE
影响因子：
5.3
作者：
GUTH, L;ZHANG, ZY;FITCH, MT
通讯作者：
FITCH, MT