Networks of gold nanoparticles and bacteriophage as biological sensors and cell-targeting agents
Networks of gold nanoparticles and bacteriophage as biological sensors and cell-targeting agents
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DOI：
10.1073/pnas.0509739103
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发表时间：
2006-01-31
影响因子：
11.1
通讯作者：
Pasqualini, R
中科院分区：
文献类型：
--
作者：
Souza, GR;Christianson, DR;Pasqualini, R
生物分子组装体是开发具有理想生物医学特性的纳米工程系统的优秀模型。在这里，我们报告了一种方法，用于制造自发的，生物活性的分子网络组成的噬菌体（噬菌体）直接组装与金（Au）纳米粒子（称为金噬菌体）。我们表明，当噬菌体工程化，使每个噬菌体颗粒显示肽，这样的网络保留细胞表面受体结合和内化属性的显示肽。这些有针对性的网络的自发组织可以进一步操纵咪唑（金噬菌体亚胺），诱导分形结构和近红外光学性质的变化。该网络可用作增强荧光和暗场显微镜，表面增强的拉曼散射检测，和近红外光-热转换的标签。总之，这些目标网络内的物理和生物特征在单个实体内提供了方便的多功能集成，具有基于纳米技术的生物医学应用的潜力。
Biological molecular assemblies are excellent models for the development of nanoengineered systems with desirable biomedical properties. Here we report an approach for fabrication of spontaneous, biologically active molecular networks consisting of bacteriophage (phage) directly assembled with gold (Au) nanoparticles (termed Au-phage). We show that when the phage are engineered so that each phage particle displays a peptide, such networks preserve the cell surface receptor binding and internalization attributes of the displayed peptide. The spontaneous organization of these targeted networks can be manipulated further by incorporation of imidazole (Au-phage-imid), which induces changes in fractal structure and near-infrared optical properties. The networks can be used as labels for enhanced fluorescence and dark-field microscopy, surf ace-enhanced Raman scattering detection, and near-infrared photon-to-heat conversion. Together, the physical and biological features within these targeted networks offer convenient multifunctional integration within a single entity with potential for nanotechnology-based biomedical applications.