ENHANCED CAROTENOID BIOSYNTHESIS BY OXIDATIVE STRESS IN ACETATE-INDUCED CYST CELLS OF A GREEN UNICELLULAR ALGA, HAEMATOCOCCUS-PLUVIALIS
ENHANCED CAROTENOID BIOSYNTHESIS BY OXIDATIVE STRESS IN ACETATE-INDUCED CYST CELLS OF A GREEN UNICELLULAR ALGA, HAEMATOCOCCUS-PLUVIALIS
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DOI：
10.1128/aem.59.3.867-873.1993
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发表时间：
1993-03-01
影响因子：
4.4
通讯作者：
NAGAI, S
中科院分区：
文献类型：
--
作者：
KOBAYASHI, M;KAKIZONO, T;NAGAI, S
在绿色的雨生红球藻（Haematococcus pluvialis）的营养生长期，加入乙酸盐或乙酸盐加Fe ~（2+），能迅速诱导其营养细胞形态转变为孢囊细胞。伴随着孢囊的形成，藻虾青素的形成更增强由添加乙酸+Fe 2+比单独添加乙酸。放线菌素D或放线菌酮抑制包囊和增强类胡萝卜素的生物合成。然而，通过单独添加乙酸盐诱导包囊形成后，即使在抑制剂的存在下，随后添加Fe 2+也可以增强类胡萝卜素的形成。Fe 2 +-增强的类胡萝卜素的合成被抑制碘化钾，羟基自由基的清除剂，这表明羟基自由基形成的铁催化的芬顿反应可能需要增强类胡萝卜素的生物合成。此外，它被证明，四个活性氧物种，单线态氧，超氧阴离子自由基，过氧化氢，和过氧自由基，能够取代Fe 2+在其作用中的增强类胡萝卜素的形成在乙酸诱导的囊肿。从这些结果中，可以得出结论，氧化应激是参与类胡萝卜素的生物合成在乙酸诱导的胞囊细胞的翻译后激活。
In a green alga, Haematococcus pluvialis, a morphological change of vegetative cells into cyst cells was rapidly induced by the addition of acetate or acetate plus Fe2+ to the vegetative growth phase. Accompanied by cyst formation, algal astaxanthin formation was more enhanced by the addition of acetate plus Fe2+ than by the addition of acetate alone. Encystment and enhanced carotenoid biosynthesis were inhibited by either actinomycin D or cycloheximide. However, after cyst formation was induced by the addition of acetate alone, carotenoid formation could be enhanced with the subsequent addition of Fe2+ even in the presence of the inhibitors. The Fe2+-enhanced carotenogenesis was inhibited by potassium iodide, a scavenger for hydroxyl radical, suggesting that hydroxyl radical formed by an iron-catalyzed Fenton reaction may be required for enhanced carotenoid biosynthesis. Moreover, it was demonstrated that four active oxygen species, singlet oxygen, superoxide anion radical, hydrogen peroxide, and peroxy radical, were capable of replacing Fe2+ in its role in the enhanced carotenoid formation in the acetate-induced cyst. From these results, it was concluded that oxidative stress is involved in the posttranslational activation of carotenoid biosynthesis in acetate-induced cyst cells.