Integrating circadian activity and gene expression profiles to predict chronotoxicity of Drosophila suzukii response to insecticides.
Integrating circadian activity and gene expression profiles to predict chronotoxicity of Drosophila suzukii response to insecticides.
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DOI：
10.1371/journal.pone.0068472
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Chiu JC
中科院分区：
文献类型：
--
作者：
Hamby KA;Kwok RS;Zalom FG;Chiu JC
Native to Southeast Asia, Drosophila suzukii (Matsumura) is a recent invader that infests intact ripe and ripening fruit, leading to significant crop losses in the U.S., Canada, and Europe. Since current D. suzukii management strategies rely heavily on insecticide usage and insecticide detoxification gene expression is under circadian regulation in the closely related Drosophila melanogaster, we set out to determine if integrative analysis of daily activity patterns and detoxification gene expression can predict chronotoxicity of D. suzukii to insecticides. Locomotor assays were performed under conditions that approximate a typical summer or winter day in Watsonville, California, where D. suzukii was first detected in North America. As expected, daily activity patterns of D. suzukii appeared quite different between ‘summer’ and ‘winter’ conditions due to differences in photoperiod and temperature. In the ‘summer’, D. suzukii assumed a more bimodal activity pattern, with maximum activity occurring at dawn and dusk. In the ‘winter’, activity was unimodal and restricted to the warmest part of the circadian cycle. Expression analysis of six detoxification genes and acute contact bioassays were performed at multiple circadian times, but only in conditions approximating Watsonville summer, the cropping season, when most insecticide applications occur. Five of the genes tested exhibited rhythmic expression, with the majority showing peak expression at dawn (ZT0, 6am). We observed significant differences in the chronotoxicity of D. suzukii towards malathion, with highest susceptibility at ZT0 (6am), corresponding to peak expression of cytochrome P450s that may be involved in bioactivation of malathion. High activity levels were not found to correlate with high insecticide susceptibility as initially hypothesized. Chronobiology and chronotoxicity of D. suzukii provide valuable insights for monitoring and control efforts, because insect activity as well as insecticide timing and efficacy are crucial considerations for pest management. However, field research is necessary for extrapolation to agricultural settings.
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影响因子：
3.8
作者：
Beaver, Laura Michelle;Hooven, Louisa Ada;Giebultowicz, Jadwiga Maria
通讯作者：
Giebultowicz, Jadwiga Maria
影响因子：
1.1
作者：
Barrozo, RB;Schilman, PE;Lazzari, CR
通讯作者：
Lazzari, CR
影响因子：
4.1
作者：
Dalton, Daniel T.;Walton, Vaughn M.;Isaacs, Rufus
通讯作者：
Isaacs, Rufus
影响因子：
64.5
作者：
Cyran, SA;Buchsbaum, AM;Blau, J
通讯作者：
Blau, J
DOI：
10.1073/pnas.0308240100
发表时间：
2004-02-17
影响因子：
11.1
作者：
Collins, BH;Rosato, E;Kyriacou, CP
通讯作者：
Kyriacou, CP