Binge pattern ethanol exposure in adolescent and adult rats: differential impact on subsequent responsiveness to ethanol.
Binge pattern ethanol exposure in adolescent and adult rats: differential impact on subsequent responsiveness to ethanol.
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DOI：
10.1111/j.1530-0277.2000.tb02091.x
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发表时间：
2000-08
期刊：
影响因子：
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通讯作者：
Aaron M. White;A. Ghia;Edward D. Levin;H. Swartzwelder
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文献类型：
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作者：
Aaron M. White;A. Ghia;Edward D. Levin;H. Swartzwelder
最近的证据表明，青春期动物比成年人更敏感的空间学习的急性乙醇暴露的破坏性影响。目前尚不清楚青春期动物是否也比成年动物对反复接触乙醇的持久神经行为影响更敏感。在这项研究中，动物在青春期或成年期以狂欢模式暴露于乙醇。当所有受试者都是成年人时，在急性乙醇挑战的存在和不存在的情况下检查空间工作记忆。方法大鼠腹腔注射乙醇（5.0g/kg）或等容生理盐水，间隔48 h，连续20 d。接触开始于出生后第30天（青少年组）或第70天（成人组）。在最后一次注射后20天，所有动物都是成年人，受试者在高架十字迷宫上进行测试，然后在八臂放射状迷宫上进行空间工作记忆任务的训练。在工作记忆任务的每次训练开始时，受试者在八只手臂中的四只手臂上取回食物奖励。延迟一段时间后，受试者被放置在迷宫中，并允许从其余四个手臂中取回食物。结果乙醇暴露对小鼠十字迷宫行为无影响。在5分钟延迟的空间工作记忆任务的采集过程中，或在随后的1小时延迟的测试过程中，各组的表现没有差异。然而，在青春期用乙醇处理的动物在乙醇挑战（1.5 g/kg腹腔注射）期间表现出比其他三组受试者更大的工作记忆障碍。结论：研究结果表明，在青春期酗酒暴露于乙醇的模式增强了对成年期乙醇记忆损害效应的反应。
BACKGROUND Recent evidence indicates that adolescent animals are more sensitive than adults to the disruptive effects of acute ethanol exposure on spatial learning. It is not yet known whether adolescent animals are also more sensitive than adults to the enduring neurobehavioral effects of repeated ethanol exposure. In this study, animals were exposed to ethanol in a binge-pattern during either adolescence or adulthood. At a time when all subjects were adults, spatial working memory was examined in the absence and presence of an acute ethanol challenge. METHODS Rats were exposed to ethanol (5.0 g/kg intraperitoneally) or isovolumetric saline at 48 hr intervals over 20 days. Exposure began on either postnatal day 30 (adolescent group) or 70 (adult group). Twenty days after the final injection, a time at which all animals were adults, the subjects were tested on an elevated plus maze and then were trained to perform a spatial working memory task on an eight-arm radial maze. At the beginning of each session of training on the working memory task, subjects retrieved food rewards on four of the eight arms. After a delay, subjects were placed on the maze and allowed to retrieve food from the remaining four arms. RESULTS Prior exposure to ethanol did not influence behavior on the plus maze. Performance of the groups did not differ during acquisition of the spatial working memory task with a 5 min delay or during subsequent testing with a 1 hr delay. However, animals treated with ethanol during adolescence exhibited larger working memory impairments during an ethanol challenge (1.5 g/kg intraperitoneally) than subjects in the other three groups. CONCLUSIONS The findings indicate that binge pattern exposure to ethanol during adolescence enhances responsiveness to the memory-impairing effects of ethanol in adulthood.