Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus.
Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus.
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DOI：
10.1038/s41598-017-04594-9
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发表时间：
2017-06-30
影响因子：
4.6
通讯作者：
Belibasakis GN
中科院分区：
文献类型：
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作者：
Bao K;Bostanci N;Thurnheer T;Belibasakis GN
双生厌氧球菌是一种相对较新发现的假定病原体，在牙周炎相关的微生物转移中具有潜在作用，牙周炎是一种导致牙周组织炎性破坏并最终导致牙齿脱落的疾病。本研究旨在介绍A. geminatus到一个多微生物生物膜模型的相关性牙周炎，并监测蛋白质组学的反应施加到其余的生物膜社区。A. geminatus与另外10个物种一起在良好建立的“龈下”体外生物膜模型中生长。通过qPCR和无标记蛋白质组学定量评估其对其他物种的影响。A.双生子引起中间青霉数量的显著增加，但不是生物膜中的其他物种。通过LC-MS/MS对生物膜进行全细胞蛋白质组分析，共鉴定出3213种蛋白质。无标记定量蛋白质组学分析表明，在A.双子肌存在于生物膜中。蛋白质表达上调和下调最多的物种是中间偃麦草和S.口腔，分别。受调节的蛋白质主要是核糖体来源的，其他受影响的类别涉及蛋白质水解，碳代谢和铁转运。总之，A.双生子可以在多微生物生物膜群落中成功生长，引起与增加的毒力特性相称的定量蛋白质组学变化。
Anaeroglobus geminatus is a relatively newly discovered putative pathogen, with a potential role in the microbial shift associated with periodontitis, a disease that causes inflammatory destruction of the periodontal tissues, and eventually tooth loss. This study aimed to introduce A. geminatus into a polymicrobial biofilm model of relevance to periodontitis, and monitor the proteomic responses exerted to the rest of the biofilm community. A. geminatus was grown together with another 10-species in a well-established “subgingival” in vitro biofilm model. Its effects on the other species were quantitatively evaluated by qPCR and label-free proteomics. A. geminatus caused a significant increase in P. intermedia numbers, but not the other species in the biofilm. Whole cell proteome profiling of the biofilms by LC-MS/MS identified a total of 3213 proteins. Label-free quantitative proteomics revealed that 187 proteins belonging to the other 10 species were differentially abundant when A. geminatus was present in the biofilm. The species with most up-regulated and down-regulated proteins were P. intermedia and S. oralis, respectively. Regulated proteins were of primarily of ribosomal origin, and other affected categories involved proteolysis, carbon metabolism and iron transport. In conclusion, A. geminatus can be successfully grown in a polymicrobial biofilm community, causing quantitative proteomic shifts commensurate with increased virulence properties.