Role of CD8α+ and CD8α- dendritic cells in the induction of primary immune responses in vivo
Role of CD8α+ and CD8α- dendritic cells in the induction of primary immune responses in vivo
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DOI：
10.1002/jlb.66.2.242
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发表时间：
1999-08-01
影响因子：
5.5
通讯作者：
Moser, M
中科院分区：
文献类型：
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作者：
Maldonado-López, R;De Smedt, T;Moser, M
来自过继转移成熟树突状细胞（DC）的数据表明它们负责诱导初级免疫。最近在脾脏中发现了DC的两个亚类，它们的表型和某些调节特征不同。在体外，两种亚群都具有激活初始T细胞的能力，尽管与CD 8 α（-）DC相比，CD 8 α（+）DC已显示出诱导T细胞凋亡和刺激较低水平的细胞因子。本研究的目的是分析这些不同的DC类型在体内的功能，我们的结果表明，这两个子集，脉冲体外抗原和注射在同基因小鼠的足垫，致敏抗原特异性T细胞的主要反应。然而，CD 8 α（+）细胞触发Th 1型细胞的发育，而CD 8 α（-）DC诱导Th 2型应答。这些观察结果表明，在体内的Th 1/Th 2平衡是由抗原呈递细胞的主要免疫反应。
Data from adoptive transfer of mature dendritic cells (DC) indicate that they are responsible for the induction of primary immunity. Two subclasses of DC have been recently identified in spleen that differ in their phenotype and in certain regulatory features. In vitro, both subsets have the capacity to activate naive T cells, although CD8 alpha(+) DC have been shown to induce T cell apoptosis and to stimulate lower levels of cytokines compared with CD8 alpha(-) DC. The objective of this study was to analyze the function of these distinct DC types in vivo, Our results show that both subsets, pulsed extracorporeally with antigen and injected in the footpads of syngeneic mice, sensitize an antigen-specific T cell primary response. However; CD8 alpha(+) cells trigger the development of Th1-type cells, whereas CD8 alpha(-) DC induce a Th2-type response. These observations suggest that the Th1/Th2 balance in,in vivo is regulated by the antigen-presenting-cells of the primary immune responses.