Abiotic Stress Resistance, a Novel Moonlighting Function of Ribosomal Protein RPL44 in the Halophilic Fungus Aspergillus glaucus
Abiotic Stress Resistance, a Novel Moonlighting Function of Ribosomal Protein RPL44 in the Halophilic Fungus Aspergillus glaucus
复制标题
非生物胁迫抗性，核糖体蛋白 RPL44 在嗜盐真菌灰绿曲霉中的新兼职功能
DOI：
10.1128/aem.00292-14
复制
发表时间：
2014-07-01
影响因子：
4.4
通讯作者：
Zhang, Shihong
中科院分区：
文献类型：
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作者：
Liu, Xiao-Dan;Xie, Lixia;Zhang, Shihong
摘要 核糖体蛋白是基底细胞器的高度保守成分，主要参与导致蛋白质合成的 mRNA 翻译。然而，某些核糖体蛋白在生物体的发育和分化中发挥作用。本研究从嗜盐真菌青曲霉（AgRPL44）中筛选出与耐盐性相关的核糖体蛋白L44（RPL44），并在酿酒酵母和烟草中研究了其活性。序列比对表明 AgRPL44 是核糖体大亚基 60S 的蛋白质之一。通过用盐、山梨醇或重金属处理，AgRPL44 的表达上调，以证明其对渗透应激的反应。克隆了来自模型真菌 Magnaporthe oryzae 的同源序列 MoRPL44，并在酵母表达系统中与 AgRPL44 进行比较。结果表明，在相似的胁迫水平下，过表达 AgRPL44 的酵母细胞比表达 MoRPL44 的酵母细胞更能抵抗盐、干旱和重金属。当AgRPL44导入米霉时，转化子表现出明显增强的耐盐和耐旱能力，表明AgRPL44在遗传应用方面具有潜在的价值。为了验证其在植物中的应用价值，用AgRPL44转化烟草，结果相似。综上所述，我们得出结论，AgRPL44 支持非生物胁迫抗性，并且可能具有遗传应用价值。
ABSTRACT Ribosomal proteins are highly conserved components of basal cellular organelles, primarily involved in the translation of mRNA leading to protein synthesis. However, certain ribosomal proteins moonlight in the development and differentiation of organisms. In this study, the ribosomal protein L44 (RPL44), associated with salt resistance, was screened from the halophilic fungus Aspergillus glaucus (AgRPL44), and its activity was investigated in Saccharomyces cerevisiae and Nicotiana tabacum. Sequence alignment revealed that AgRPL44 is one of the proteins of the large ribosomal subunit 60S. Expression of AgRPL44 was upregulated via treatment with salt, sorbitol, or heavy metals to demonstrate its response to osmotic stress. A homologous sequence from the model fungus Magnaporthe oryzae, MoRPL44, was cloned and compared with AgRPL44 in a yeast expression system. The results indicated that yeast cells with overexpressed AgRPL44 were more resistant to salt, drought, and heavy metals than were yeast cells expressing MoRPL44 at a similar level of stress. When AgRPL44 was introduced into M. oryzae, the transformants displayed obviously enhanced tolerance to salt and drought, indicating the potential value of AgRPL44 for genetic applications. To verify the value of its application in plants, tobacco was transformed with AgRPL44, and the results were similar. Taken together, we conclude that AgRPL44 supports abiotic stress resistance and may have value for genetic application.