Protective Effects of Kaempferol on Lipopolysaccharide-Induced Mastitis in Mice
Protective Effects of Kaempferol on Lipopolysaccharide-Induced Mastitis in Mice
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DOI：
10.1007/s10753-014-9870-9
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发表时间：
2014-10-01
期刊：
影响因子：
5.1
通讯作者：
Zhang, Naisheng
中科院分区：
文献类型：
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作者：
Cao, Rongfeng;Fu, Kaiqiang;Zhang, Naisheng
山柰酚是从菊科植物高良姜和其他中草药的根中分离出来的，据报道具有抗炎作用。然而，山奈酚对脂多糖（LPS）诱导的乳腺炎的抗炎作用尚不清楚，其潜在的分子机制仍有待探讨。本研究的目的是评价山奈酚对LPS诱导的小鼠乳腺炎的影响。通过乳腺导管注射LPS建立小鼠乳腺炎模型。在LPS诱导前1 h和诱导后12 h腹腔注射山奈酚。目前的结果表明，山奈酚显着减少嗜中性粒细胞浸润，髓过氧化物酶（MPO）的激活，肿瘤坏死因子-α（TNF-α），白细胞介素-6（IL-6），白细胞介素-1 β（IL-1 β）的表达，在剂量依赖性的方式，增加LPS诱导的小鼠乳腺炎。此外，山奈酚抑制核因子-κ B（NF-κ B）p65亚基的磷酸化及其抑制剂I κ B α的降解。提示山奈酚可能通过抑制NF-κ B信号通路发挥抗炎作用。山奈酚可能是一种潜在的乳腺炎治疗药物。
Kaempferol isolated from the root of Zingiberaceae plants galangal and other Chinese herbal medicines have been reported to have anti-inflammatory properties. However, the anti-inflammatory effects of kaempferol on lipopolysaccharide (LPS)-induced mastitis are unknown and their underlying molecular mechanisms remain to be explored. The aim of this study was to evaluate the effects of kaempferol on LPS-induced mouse mastitis. The mouse model of mastitis was induced by injection of LPS through the duct of mammary gland. Kaempferol was injected 1 h before and 12 h after induction of LPS intraperitoneally. The present results showed that kaempferol markedly reduced infiltration of neutrophilic granulocyte, activation of myeloperoxidase (MPO), expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and interleukin-1 beta (IL-1 beta) in a dose-dependent manner, which were increased in LPS-induced mouse mastitis. Furthermore, kaempferol suppressed the phosphorylation of nuclear factor-kappa B (NF-kappa B) p65 subunit and the degradation of its inhibitor I kappa B alpha. All results suggest that anti-inflammatory effects of kaempferol against the LPS-induced mastitis possibly through inhibition of the NF-kappa B signaling pathway. Kaempferol may be a potential therapeutic agent for mastitis.