SITE AND CHARACTERISTICS OF ELECTROLYTE LOSS AND EFFECT OF INTRALUMINAL GLUCOSE IN EXPERIMENTAL CANINE CHOLERA
SITE AND CHARACTERISTICS OF ELECTROLYTE LOSS AND EFFECT OF INTRALUMINAL GLUCOSE IN EXPERIMENTAL CANINE CHOLERA
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DOI：
10.1172/jci105810
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发表时间：
1968-01-01
影响因子：
15.9
通讯作者：
STEENBERG, RW
中科院分区：
文献类型：
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作者：
CARPENTER, CC;SACK, RB;STEENBERG, RW
对8只经口胃给药6小时诱发实验性霍乱的狗进行胃肠道电解质丢失的部位和特征的研究。霍乱弧菌小川 395 株肉汤培养物。在这些动物中，所有电解质损失均源自小肠，主要来自空肠和回肠。小肠液的碳酸氢盐浓度显示出从十二指肠到回肠末端进行性增加，十二指肠的碳酸氢盐浓度低于血浆的浓度，回肠末端的碳酸氢盐浓度显着高于同时获得的血浆的浓度。对慢性 Thiry-Vella 空肠环（5 只狗）和慢性 Thiry-Vella 回肠环（5 只狗）对霍乱外毒素腔内攻击的反应的研究表明，所有环都表现出等渗电解质损失 14-18 小时。挑战后的一段时间。受外毒素攻击的空肠环产生的液体的碳酸氢盐浓度与血浆的没有显着差异，而回肠环产生的液体的碳酸氢盐浓度约为血浆的3倍。 12. 通过对患有慢性 Thiry-Vella 瘘管的狗进行灌注研究，研究了腔内葡萄糖对犬肠道对霍乱外毒素反应的影响。腔内葡萄糖显着增强空肠和回肠环中的等渗液体吸收。粗制霍乱外毒素腔内给药前后，葡萄糖对等渗液体吸收的净影响相等。霍乱外毒素可能通过一种与葡萄糖增强钠吸收无关的机制导致肠道电解质损失。
The site and character-istics of gastrointestinal electrolyte loss were investigated in 8 dogs with experimental cholera induced by orogastric administration of 6-hr. broth cultures of Vibrio cholerae, strain Ogawa 395. In these animals, all electrolyte losses originated in the small bowel, predominantly from the jejunum and ileum. The bicarbonate concentration of the small bowel fluid showed a progressive increase from duodenum, where it was less than that of plasma, to the terminal ileum, where it was significantly greater than that of simultaneously obtained plasma. Studies of the responses of chronic Thiry-Vella jejunal loops (5 dogs) and chronic Thiry-Vella ileal loops (5 dogs) to intraluminal challenge by cholera exotoxin demonstrated the all loops exhibited isotonic electrolyte loss for a 14-18 hr. period after challenge. The bicarbonate concentration of fluid produced by exotoxin-challenged jejunal loops was not significantly different from that of plasma, whereas the ileal loops produced fluid with a bicarbonate concentration approximately 3 times that of plasma. The effect of intraluminal glucose on the response of canine gut to cholera exotoxin was investigated by perfusion studies in 12. dogs with chronic Thiry-Vella fistulae. Intraluminal glucose significantly enhanced isotonic fluid absorption in both jejunal and ileal loops. The net effects of glucose on isotonic fluid absorption were equal before and after intraluminal administration of crude cholera exotoxin. Cholera exotoxin may cause gut electrolyte loss by a mechanism independent of that by which glucose enhances Na absorption.