SIMPLE DETERMINATION OF ACETYLCHOLINE AND CHOLINE WITHIN 4 MIN BY HPLC-ECD AND IMMOBILIZED ENZYME COLUMN IN MICE BRAIN-AREAS
SIMPLE DETERMINATION OF ACETYLCHOLINE AND CHOLINE WITHIN 4 MIN BY HPLC-ECD AND IMMOBILIZED ENZYME COLUMN IN MICE BRAIN-AREAS
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DOI：
10.1016/0160-5402(89)90063-6
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发表时间：
1989-07-01
期刊：
影响因子：
--
通讯作者：
ITOH, T
中科院分区：
文献类型：
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作者：
MURAI, S;MIYATE, H;ITOH, T
本文描述了一种使用高效液相色谱 (HPLC) 与电化学检测 (ECD) 和固定化酶柱相结合的快速而简单的方法，用于同时测定小鼠离散脑区域中的乙酰胆碱 (ACh) 和胆碱 (Ch)。将小脑组织的高氯酸提取物直接注射到 HPLC 系统中，无需预净化程序。每次色谱运行均在 4 分钟内测定 ACh 和 Ch 水平。回收率为 100.0.+-。乙酰胆碱为 4.8%，96.1 .+-。 Ch 为 1.4%（平均值 .+-.S.D.，n = 10）两种化合物的检测限均为 0.25 pmol（信噪比为 3）。本方法已应用于小鼠几个离散脑区 Ch 和 ACh 的测量。
A rapid and simple method using a combination of high-performance liquid chromatography (HPLC) with electrochemical detection (ECD) and an immobilized enzyme column herein is described for simultaneous assay of acetylcholine (ACh) and choline (Ch) in discrete brain areas of the mouse. Perchloric acid extracts of small brain tissues were injected directly onto the HPLC system with no precleanup procedure. The ACh and Ch levels were determined within 4 min for each chromatographic run. Recoveries were 100.0 .+-. 4.8% for ACh and 96.1 .+-. 1.4% (mean .+-. S.D., n = 10) for Ch. Detection limits for both compounds were 0.25 pmol (signal-to-noise ratio of three). The present method has been applied to the measurement of Ch and ACh in several discrete brain areas of the mouse.