IMMUNOCYTOCHEMICAL LOCALIZATION OF CHOLINE-ACETYLTRANSFERASE WITHIN THE RAT NEOSTRIATUM - A CORRELATED LIGHT AND ELECTRON-MICROSCOPIC STUDY OF CHOLINERGIC NEURONS AND SYNAPSES
IMMUNOCYTOCHEMICAL LOCALIZATION OF CHOLINE-ACETYLTRANSFERASE WITHIN THE RAT NEOSTRIATUM - A CORRELATED LIGHT AND ELECTRON-MICROSCOPIC STUDY OF CHOLINERGIC NEURONS AND SYNAPSES
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DOI：
10.1002/cne.902380305
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发表时间：
1985-01-01
影响因子：
2.5
通讯作者：
VAUGHN, JE
中科院分区：
文献类型：
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作者：
PHELPS, PE;HOUSER, CR;VAUGHN, JE
胆碱乙酰转移酶（ChAT）的单克隆抗体被用于免疫细胞化学研究，以表征假定的胆碱能神经元和突触连接在大鼠尾壳核。光学显微镜（LM）显示，ChAT阳性神经元分布在整个纹状体。这些细胞有大的椭圆形或多极胞体，并显示3-4个初级树突，分支并延伸很长的距离。复染制剂的定量分析表明，ChAT阳性神经元占总神经元群体的1.7%。EM的免疫反应神经元最初研究LM显示胞体的特点是深深内陷的细胞核和丰富的细胞器丰富的细胞质。ChAT阳性神经元的表面通常是光滑的，但偶尔会出现躯体突起和树突棘。虽然很少观察到，ChAT阳性神经元的轴突分支，接受突触，并成为髓鞘。未标记的终扣与ChAT阳性胞体和近端树突形成对称和不对称突触，但在远端树突上数量较多。一些未标记的终末与ChAT阳性胞体和树突形成不对称突触，突触下致密体突出。光学显微镜观察显示，ChAT阳性纤维和点状结构在纹状体的密集分布，这些结构似乎相关，分别与标记的终末前轴突和突触前终扣识别EM。ChAT阳性终扣含有多形性小泡，主要与未标记的树突轴形成对称突触。它们与超微结构类似于中型多刺神经元的未标记神经元的胞体、树突和轴突起始段建立突触联系。中型棘状GABA能投射神经元显然接受胆碱能神经支配，这可能是来自ChAT阳性纹状体细胞。尾壳核内的胆碱能神经元显然属于一个单一的细胞群体，具有大的胞体和广泛的稀疏的树突。这样的神经元，在与ChAT阳性纤维和终端的密集浓度相结合，是可能的基础上，大量的ChAT和乙酰胆碱检测生化内的新纹状体。
Monoclonal antibodies to choline acetyltransferase (ChAT) were used in an immunocytochemical study to characterize putative cholinergic neurons and synaptic junctions in rat caudate-putamen. Light microscopy (LM) revealed that ChAT-positive neurons are distributed throughout the striatum. These cells have large oval or multipolar somata and exhibit 3-4 primary dendrites that branch and extend long distances. Quantitative analysis of counterstained preparations indicated that ChAT-positive neurons constitute 1.7% of the total neuronal population. EM of immunoreactive neurons initially studied by LM revealed somata characterized by deeply invaginated nuclei and by abundant amounts of organelle-rich cytoplasm. Surfaces of ChAT-positive neurons are frequently smooth, but occasional somatic protrusions and dendritic spines occur. Although infrequently observed, axons of ChAT-positive neurons branch, receive synapses and become myelinated. Unlabeled boutons make symmetrical and asymmetrical synapses with ChAT-positive somata and proximal dendrites, but are more numerous on distal dendrites. Some unlabeled terminals form asymmetrical synapses with ChAT-positive somata and dendrites that are distinguished by prominent subsynaptic dense bodies. Light microscopy demonstrated a dense distribution of ChAT-positive fibers and punctate structures in the striatum; these structures appear to correlate, respectively, with labeled preterminal axons and presynaptic boutons identified by EM. ChAT-positive boutons contain pleomorphic vesicles and make symmetrical synapses primarily with unlabeled dendritic shafts. They establish synaptic contacts with somata, dendrites and axon initial segments of unlabeled neurons that ultrastructurally resemble medium spiny neurons. Medium spiny GABAergic projection neurons evidently receive a cholinergic innervation that is probably derived from ChAT-positive striatal cells. Cholinergic neurons within caudate-putamen evidently belong to a single population of cells that have large somata and extensive sparsely spined dendrites. Such neurons, in combination with dense concentrations of ChAT-positive fibers and terminals, are the likely basis for the large amounts of ChAT and acetylcholine detected biochemically within the neostriatum.