Regulation of Na+, K(+)-ATPase by chronic ethanol exposure of PC 12 cells.
Regulation of Na+, K(+)-ATPase by chronic ethanol exposure of PC 12 cells.
复制标题
PC 12 细胞慢性乙醇暴露对 Na , K( )-ATP 酶的调节。
DOI：
10.1016/0006-2952(93)90306-h
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发表时间：
1993
影响因子：
5.8
通讯作者：
Acara,MA
中科院分区：
文献类型：
--
作者：
Rabin,RA;Acara,MA
The effects of chronic ethanol exposure on Na+,K+-ATPase were investigated in PC 12 cells. Inclusion of ethanol in the Na+,K+-ATPase assay (i.e.in vitroaddition of ethanol) inhibited enzyme activity. Conversely, intrinsic Na+,K+-ATPase activity was increased after chronic ethanol exposure of the cells. This increase in Na+,K+pumps occurred without any alteration in the inhibitory effects ofin vitroethanol. A similar response was observed when the chronic treatments were carried out using serum-free defined medium. The effects of other agents, which like ethanol decrease membrane order, were investigated. The addition of ketamine andtert-butanolin vitrocaused a concentration-dependent inhibition of Na+,K+-ATPase activity. However, chronic exposure of the PC 12 cells totert-butanol or ketamine did not alter either intrinsic Na+,K+-ATPase activity or the inhibitory effects of ethanolin vitro. Maintenance of PC 12 cells in medium containing ethanol resulted in an increase in the intracellular content of Na+without any change in the K+levels. In contrast, maintenance of the cells in medium containingtert-butanol did not alter intracellular levels of Na+or K+. The present study shows that the ethanol-induced increase in Na+,K+pumps involved an increase in the intracellular content of Na+. This increase in Na+content did not appear to be secondary to an inhibition of Na+,K+-ATPase activity.
