ANALYSIS OF HISTONE GENE-EXPRESSION DURING THE CELL-CYCLE IN HELA-CELLS BY USING CLONED HUMAN HISTONE GENES
ANALYSIS OF HISTONE GENE-EXPRESSION DURING THE CELL-CYCLE IN HELA-CELLS BY USING CLONED HUMAN HISTONE GENES
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DOI：
10.1073/pnas.79.3.749
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
STEIN, G
中科院分区：
文献类型：
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作者：
RICKLES, R;MARASHI, F;STEIN, G
组蛋白的合成显然局限于细胞周期的S期，因此与DNA复制平行。转录和转录后水平的控制都被调用。以代表不同组蛋白基因簇的几个克隆的人类组蛋白基因组序列为探针，采用印迹杂交技术，对G1期和S期HeLa S3细胞的细胞核和细胞质中组蛋白RNA的稳态水平进行了评估。与S期细胞相比，G1期细胞的组蛋白mRNA序列在细胞质中的代表性< 1%，1%在核内。这些数据与转录调控一致，但不能完全排除组蛋白基因表达调控在某种程度上是转录后介导的可能性。如果组蛋白基因转录确实发生在G1期，则RNA必须要么快速降解，要么与S期相比转录到有限的程度。当来自G1和S期细胞的胞质RNA的印迹与λ HHG 41 DNA（含有H3和H4人基因组蛋白序列）杂交时，获得了意想不到的结果。虽然在S期的RNA中观察到与组蛋白mRNA的杂交，但在G1期的RNA中没有观察到与组蛋白mRNA的杂交，但在G1期的RNA中观察到与非组蛋白RNA的杂交。330个核苷酸主要存在于G1中。
Histone protein synthesis is apparently restricted to the S phase of the cell cycle and therefore parallels DNA replication.sbd.both transcriptional and posttranscriptional levels of control were invoked. Using blot hybridization with several cloned genomic human histone sequences representing different histone gene clusters as probes, the steady-state level of histone RNA was assessed in the nucleus and cytoplasm of G1 and S phase HeLa S3 cells. The representation of histone mRNA sequences of G1 compared with S phase cells was < 1% in the cytoplasm and .apprxeq. 1% in the nucleus. These data are consistent with transcriptional control, but do not completely dismiss the possibility that regulation of histone gene expression is, to some extent, mediated posttranscriptionally. If histone gene transcription does occur in G1, the RNA must either be rapidly degraded or be transcribed to a limited extent compared with S phase. An unexpected result was obtained when a blot of cytoplasmic RNA from G1 and S phase cells was hybridized with .lambda.HHG 41 DNA (containing H3 and H4 human genomic histone sequences). Although hybridization with histone mRNA was observed for RNA from S phase but not from G1 cells, hybridization with a nonhistone RNA of .apprxeq. 330 nucleotides present predominantly in G1 was also observed.