Structure and mechanism of DNA topoisomerase II
Structure and mechanism of DNA topoisomerase II
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DOI：
10.1038/379225a0
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发表时间：
1996-01-18
期刊：
影响因子：
64.8
通讯作者：
Wang, JC
中科院分区：
文献类型：
--
作者：
Berger, JM;Gamblin, SJ;Wang, JC
酵母 II 型 DNA 拓扑异构酶大片段的晶体结构揭示了具有大中心孔的心形二聚体蛋白。它提供了酶的分子模型，作为 ATP 调节的夹子，两端有两组钳口，通过多个关节连接。结合 DNA 的酶可以通过一组钳口接纳第二个 DNA 双链体，将其运输通过切割的第一个双链体，并通过另一组钳口将其排出。
The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints. An enzyme with bound DNA can admit a second DNA duplex through one set of jaws, transport it through the cleaved first duplex, and expel it through the other set of jaws.