Screening of natural compounds with neuronal differentiation promoting effects in a cell-based model.
Screening of natural compounds with neuronal differentiation promoting effects in a cell-based model.
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DOI：
10.1016/s1875-5364(15)30056-x
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发表时间：
2015-08-01
影响因子：
4.6
通讯作者：
Liao, Hong
中科院分区：
文献类型：
--
作者：
Chen, Tao;Wang, Juan;Liao, Hong
本研究的目的是以P19胚胎癌细胞为细胞模型，建立具有神经元分化促进作用的中药小分子药物筛选方法。首先将构建的质粒（pTalpha1-Luc）转染P19细胞，建立筛选模型。其次，利用建立的模型和全反式维A酸作为阳性对照，筛选了几种中药。最后，利用免疫荧光染色、qT-PCR 和蛋白质印迹分析探讨了潜在的分子机制。结果表明，药物筛选模型成功建立，和厚朴酚和金丝桃苷均可诱导P19分化为神经元，其可能的分子机制是调节Wnt信号通路。总之，本研究开发的药物筛选模型提供了一个快速的、基于细胞的筛选平台，用于鉴定具有神经元分化作用的天然化合物。
The purpose of this study was to establish a drug screening method for small molecules extracted from traditional Chinese medicines (TCM) that have neuronal differentiation promoting effects, using P19 embryonic carcinoma cell as a cell-based model. First, the constructed plasmid (pTalpha1-Luc) was transfected into P19 cells to establish a screening model. Second, several TCMs were screened using the established model and all-trans-retinoic acid as a positive control. Finally, the underlying molecular mechanism was explored using immunofluorescence staining, qT-PCR, and Western blot analysis. Our results indicated that the drug screen model was established successfully and that both honokiol and hyperoside induced P19 differentiation into neurons, with the possible molecular mechanism being modulating the Wnt signaling pathway. In conclusion, the drug screening model developed in the present study provides a rapid, cell-based screening platform for identifying natural compounds with neuronal differentiation effects.