Antibody-induced redistribution of CEA on the cell surface: utilization in separation of CEA and isoantigen A.
Antibody-induced redistribution of CEA on the cell surface: utilization in separation of CEA and isoantigen A.
复制标题
抗体诱导的 CEA 在细胞表面的重新分布：用于分离 CEA 和同种抗原 A。
DOI：
10.4049/jimmunol.115.4.1049
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发表时间：
1975
影响因子：
4.4
通讯作者：
W. Tompkins
中科院分区：
文献类型：
--
作者：
K. Rosenthal;J. L. Palmer;J. Harris;W. Rawls;W. Tompkins
癌胚抗原 (CEA) 特异性抗体诱导 CEA 的极性重新分布，CEA 在组织培养物中生长的人肠癌细胞表面表达。极性重新分布发生在 23°C 和 37°C，但在 4°C 时不发生，并被叠氮化钠和细胞松弛素 B 抑制。同种抗原 A 的抗体不与细胞表面的 CEA 反应，但与未被抗体重新分布的同种抗原 A 反应。使用同时表达CEA和同种抗原A的细胞来证明可以在不改变同种抗原A的分布的情况下诱导CEA的极性重新分布。数据表明CEA和同种抗原A可以作为单独的分子在同一细胞上表达。
Antibodies specific for carcinoembryonic antigen (CEA) induced polar redistribution of CEA which was expressed on the surface of human intestinal carcinoma cells grown in tissue culture. Polar redistribution occurred at 23 degrees C and 37 degrees C but not at 4 degrees C and was inhibited by sodium azide and cytochalasin B. Antibodies to isoantigen A did not react with CEA at the cell surface but did react with isoantigen A which was not redistributed by the antibody. Cells which expressed both CEA and isoantigen A were used to demonstrate that polar redistribution of CEA could be induced without altering the distribution of isoantigen A. The data indicate that CEA and isoantigen A can be expressed on the same cell as separate molecules.