A "Turn-On" Fluorescent Sensor for Methylglyoxal
A "Turn-On" Fluorescent Sensor for Methylglyoxal
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DOI：
10.1021/ja406077j
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发表时间：
2013-08-21
影响因子：
15
通讯作者：
Spiegel, David A.
中科院分区：
文献类型：
--
作者：
Wang, Tina;Douglass, Eugene F., Jr.;Spiegel, David A.
甲基乙二醛（MGO）是所有活细胞产生的二羰基代谢物，与许多人类疾病有关。然而，由于缺乏监测和检测MGO的直接方法，对其生物学作用的研究一直受到阻碍。为了解决这一限制，我们开发了一种荧光传感器（甲基二氨基苯- bodipy，或“MBo”），可以在生理条件下检测MGO。我们发现MBo对MGO的选择性优于其他生物相关的二羰基，适合在复杂的环境中检测MGO，包括活细胞。此外，我们证明了MBo在估计血浆中MGO浓度方面的效用。本文报道的结果有可能推进临床和基础科学研究和实践。
Methylglyoxal (MGO), a dicarbonyl metabolite produced by all living cells, has been associated with a number of human diseases. However, studies of the role(s) MGO plays biologically have been handicapped by a lack of direct methods for its monitoring and detection. To address this limitation, we have developed a fluorescent sensor (methyl diaminobenzene-BODIPY, or "MBo") that can detect MGO under physiological conditions. We show that MBo is selective for MGO over other biologically relevant dicarbonyls and is suitable for detecting MGO in complex environments, including that of living cells. In addition, we demonstrate MBo's utility in estimating plasma concentrations of MGO. The results reported herein have the potential to advance both clinical and basic science research and practice.