A Rapid Alkalinization Factor-like Peptide EaF82 Impairs Tapetum Degeneration during Pollen Development through Induced ATP Deficiency.
A Rapid Alkalinization Factor-like Peptide EaF82 Impairs Tapetum Degeneration during Pollen Development through Induced ATP Deficiency.
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DOI：
10.3390/cells12111542
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发表时间：
2023-06-04
期刊：
影响因子：
6
通讯作者：
Xie, Jiahua
中科院分区：
文献类型：
--
作者：
Hung, Chiu-Yueh;Kittur, Farooqahmed S.;Wharton, Keely N.;Umstead, Makendra L.;Burwell, D'Shawna B.;Thomas, Martinique;Qi, Qi;Zhang, Jianhui;Oldham, Carla E.;Burkey, Kent O.;Chen, Jianjun;Xie, Jiahua
关键词：
在植物中，绒毡层细胞的及时退化对于提供营养和其他物质以支持花粉发育至关重要。快速碱化因子（RALF）是一类富含半胱氨酸的小分子多肽，参与植物生长发育的各个方面，以及抵御生物和非生物胁迫。然而，它们中的大多数的功能仍然是未知的，而没有RALF已被报道涉及绒毡层退化。在这项研究中，我们证明了一种新的富含半胱氨酸的肽，EaF 82，从害羞开花的'黄金Pothos'（Epipremnum aureum）植物分离，是一个RALF样肽，并显示碱化活性。它在拟南芥中的异源表达延缓了绒毡层退化，降低了花粉产量和种子产量。RNAseq、RT-qPCR和生化分析表明，EaF 82的过表达下调了一组涉及pH变化、细胞壁修饰、绒毡层退化和花粉成熟的基因，以及7个内源拟南芥RALF基因，并降低了蛋白酶体活性和ATP水平。酵母双杂交筛选确定AKIN 10，能量敏感SnRK 1激酶的亚基，作为其相互作用的伴侣。我们的研究揭示了RALF肽在绒毡层退化中可能的调节作用，并表明EaF 82的作用可能通过AKIN 10介导，导致转录组和能量代谢的改变，从而导致ATP缺乏，损害花粉发育。
In plants, the timely degeneration of tapetal cells is essential for providing nutrients and other substances to support pollen development. Rapid alkalinization factors (RALFs) are small, cysteine-rich peptides known to be involved in various aspects of plant development and growth, as well as defense against biotic and abiotic stresses. However, the functions of most of them remain unknown, while no RALF has been reported to involve tapetum degeneration. In this study, we demonstrated that a novel cysteine-rich peptide, EaF82, isolated from shy-flowering ‘Golden Pothos’ (Epipremnum aureum) plants, is a RALF-like peptide and displays alkalinizing activity. Its heterologous expression in Arabidopsis delayed tapetum degeneration and reduced pollen production and seed yields. RNAseq, RT-qPCR, and biochemical analyses showed that overexpression of EaF82 downregulated a group of genes involved in pH changes, cell wall modifications, tapetum degeneration, and pollen maturation, as well as seven endogenous Arabidopsis RALF genes, and decreased proteasome activity and ATP levels. Yeast two-hybrid screening identified AKIN10, a subunit of energy-sensing SnRK1 kinase, as its interacting partner. Our study reveals a possible regulatory role for RALF peptide in tapetum degeneration and suggests that EaF82 action may be mediated through AKIN10 leading to the alteration of transcriptome and energy metabolism, thereby causing ATP deficiency and impairing pollen development.
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影响因子：
4
作者：
De Smet, Ive;Juergens, Gerd
通讯作者：
Juergens, Gerd
影响因子：
7.4
作者：
Covey, Paul A.;Subbaiah, Chalivendra C.;Bedinger, Patricia A.
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Bedinger, Patricia A.
DOI：
10.1042/bcj20160792
发表时间：
2017-02-01
期刊：
The Biochemical journal
影响因子：
--
作者：
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通讯作者：
Ferguson KM
影响因子：
11.4
作者：
Gómez JF;Talle B;Wilson ZA
通讯作者：
Wilson ZA
影响因子：
11.4
作者：
Farrás, R;Ferrando, A;Koncz, C
通讯作者：
Koncz, C