Electrochemical sensor for enzymatic lactate detection based on laser-scribed graphitic carbon modified with platinum, chitosan and lactate oxidase
Electrochemical sensor for enzymatic lactate detection based on laser-scribed graphitic carbon modified with platinum, chitosan and lactate oxidase
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DOI：
10.1016/j.talanta.2022.123492
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发表时间：
2022-08-15
期刊：
影响因子：
6.1
通讯作者：
Teixeira, Sofia Rodrigues
中科院分区：
文献类型：
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作者：
Madden, Julia;Vaughan, Eoghan;Teixeira, Sofia Rodrigues
我们开发了一种灵活的激光划片石墨碳基乳酸盐生物传感器制造使用低成本的450 nm激光。我们展示了一种简单的制造方法，包括电沉积铂，然后用壳聚糖和乳酸氧化酶进行改性的两个铸造步骤。该生物传感器表现出在0.2 mM至3 mM的线性范围内（R-2 > 0.99）的计时电流法乳酸检测，检测限为0.11 mM，灵敏度为35.8 μ A/mM/cm（2）。该生物传感器成功地进行了多达10次连续测量（一个接一个），表明良好的工作稳定性（RSD
We developed a flexible laser scribed graphitic carbon based lactate biosensor fabricated using a low cost 450 nm laser. We demonstrated a facile fabrication method involving electrodeposition of platinum followed by two casting steps for modification with chitosan and lactate oxidase. The biosensor demonstrated chronoamperometric lactate detection within a linear range from 0.2 mM to 3 mM, (R-2 > 0.99), with a limit of detection of 0.11 mM and a sensitivity of 35.8 mu A/mM/cm(2). The biosensor was successful in performing up to 10 consecutive measurements (one after the other) indicating good working stability (RSD