Phase Fluorometry Using a Continuously Modulated Laser Diode.
Phase Fluorometry Using a Continuously Modulated Laser Diode.
复制标题
使用连续调制激光二极管的相位荧光测定法。
DOI:
10.1021/ac00042a009
复制
发表时间:
1992
影响因子:
7.4
通讯作者:
Lakowicz,JosephR
中科院分区:
文献类型:
--
作者:
Thompson,RichardB;Frisoli,JoanK;Lakowicz,JosephR
The authors report phase fluorometrlc measurements using directly amplitude-modulated 670-and 791-nm laser diodes as the excitation source. Fluorescence lifetimes ranging from 220 ps to 1.7 ns were measured using a commercial phase fluorometer. It was found that higher amounts of radio frequency power were required to achieve comparable degrees of modulation when the dc current on the diode was Increased and that rf power above a certain level created distortion In the light output. Results are shown for common cyanine laser dyes emitting at wavelengths to 840 nm.