Sucrase metabolism in germfree rats.
Sucrase metabolism in germfree rats.
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无菌大鼠中的蔗糖酶代谢。
DOI：
10.1152/ajpgi.1982.242.6.g650
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Korsmo,HA
中科院分区：
文献类型：
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作者：
Olsen,WA;Korsmo,HA
我们研究了无菌动物、前无菌动物和常规对照中肠道刷状缘蛋白蔗糖酶-异麦芽糖酶的降解，以确定肠道细菌改变双糖酶水平的机制。我们的结果表明，无菌动物中蔗糖酶-异麦芽糖酶的周转与其他组中一样快，并且与生理条件下胰腺蛋白酶对表面二糖酶的去除一致。我们的结果还表明，无菌动物中二糖酶水平升高在很大程度上是成熟肠上皮细胞数量增加的结果，在动物暴露于常规微生物菌群后，这种现象持续至少2周。
We studied degradation of the intestinal brush-border protein sucrase-isomaltase in germfree animals as well as in ex-germfree animals and conventional controls to determine the mechanism by which intestinal bacteria alter disaccharidase levels. Our results indicate that sucrase-isomaltase turnover is as rapid in germfree animals as in the other groups and are consistent with surface removal of disaccharidases by pancreatic proteases under physiological conditions. Our results also suggest that elevated disaccharidase levels in germfree animals are in large part the consequence of an increased number of mature enterocytes, which persists for at least 2 wk after exposure of animals to a conventional microbial flora.