Effects of Crocin on Spatial Memory Impairment Induced by Hyoscine and Its Effects on BDNF, CREB, and p-CREB Protein and mRNA Levels in Rat Hippocampus
Effects of Crocin on Spatial Memory Impairment Induced by Hyoscine and Its Effects on BDNF, CREB, and p-CREB Protein and mRNA Levels in Rat Hippocampus
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DOI：
10.5812/jjnpp.64315
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发表时间：
2017-08-01
影响因子：
0.6
通讯作者：
Hosseinzadeh, Hossein
中科院分区：
文献类型：
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作者：
Behravanfar, Nima;Abnous, Khalil;Hosseinzadeh, Hossein
背景：番红花 (Crocus sativus L., (Iridaceace)，又名藏红花，在传统医学中用于不同目的，例如改善记忆力。藏红花素是藏红花许多药用特性的药理活性成分之一。 目的：在本研究中，藏红花活性成分藏红花素对东莨菪碱诱导的记忆障碍的影响及其对 CREB（cAMP 反应元件结合蛋白）、BDNF（脑源性神经营养因子）和 BDNF（脑源性神经营养因子）的蛋白质和 mRNA 转录水平的影响 在大鼠海马中研究了磷酸-CREB (p-CREB)。 方法：在给予东莨菪碱 (1 mg/kg) 前 30 分钟，对大鼠腹腔注射藏红花素 (10、20 和 40 mg/kg)、生理盐水 (1 mL/kg) 和卡巴拉汀 (1.5 mg/kg)，持续 5 天。使用莫里斯水迷宫研究了其对空间学习和记忆的影响。分别使用蛋白质印迹和 qRT PCR 方法分析 CREB 和 BDNF 的蛋白质和转录水平。 结果：与东莨菪碱相比，用藏红花素（20 和 40 mg/kg）和卡巴拉汀治疗 5 天，在第 8 天的探测试验中显着增加了目标象限中动物的存在。与东莨菪碱相比，藏红花素（20 mg/Kg）显着增加了 BDNF 和 CREB 蛋白和转录水平。结论：在莫里斯水迷宫试验中，藏红花素通过减少寻找平台的潜伏期，改善了东莨菪碱引起的记忆障碍。藏红花素改善记忆力可能是由于 BDNF 和 CREB 蛋白和转录水平的增加。
Background: Crocus sativus L., (Iridaceace), known as saffron, is used in traditional medicine for different purposes such asmemory improvment. Crocin is one of the pharmacologically active constituents responsible for many medicinal properties of saffron.Objectives: In this study, the effects of crocin, an active saffron constituent, on hyoscine inducedmemory impairment and its effect onprotein andmRNAtranscript levels of CREB (cAMP response element binding protein), BDNF(brain-derived neurotrophic factor), and phospho-CREB (p-CREB) were investigated in rat hippocampus.Methods: Rats were intraperitoneally treated with crocin (10, 20, and 40 mg/kg), normal saline (1 mL/kg), and rivastigmine (1.5 mg/kg) 30 minutes prior to hyoscine (1 mg/kg) for five days. The effects were studied on spacial learning and memory using Morris water maze. Protein and transcript levels of CREB and BDNF were analyzed using western blotting and qRT PCR methods, respectively.Results: Five days treatment with crocin (20 and 40 mg/kg) and rivastigmine significantly increased the presence of animals in target quadrant in probe trial on the 8th day compared to hyoscine. Crocin (20 mg/Kg) increased significantly BDNF and CREB protein and transcript levels compared to hyoscine.Conclusions: Crocin improved memory impairment induced by hyoscine through reduction of the latency to find the platform in the probe trial in Morris water maze test. Crocin improved memory probably due to the increase of BDNF and CREB protein and transcript levels.