Comparative study of sulfite pretreatments for robust enzymatic saccharification of corn cob residue.
Comparative study of sulfite pretreatments for robust enzymatic saccharification of corn cob residue.
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亚硫酸盐预处理对玉米芯渣酶促糖化的比较研究
DOI：
10.1186/1754-6834-5-87
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发表时间：
2012-12-04
影响因子：
6.3
通讯作者：
Jiang J
中科院分区：
文献类型：
--
作者：
Bu L;Xing Y;Yu H;Gao Y;Jiang J
玉米芯残渣(CCR)是一种废弃的木质纤维原料，具有巨大的生物乙醇生产潜力。采用温和的亚硫酸盐工艺，通过磺化和水解来增强材料的亲水性。用化学组成、FT-IR光谱和电导滴定表征了不同亚硫酸盐处理环境下Ccr的变化。结果在中性、酸性、碱性和乙醇环境中，亚硫酸盐处理使CCR中的木质素含量(43.2%)分别下降到37.8%、38.0%、35.9%和35.5%。亚硫酸盐处理提高了CCR的产糖量。在糖化样品中，乙醇亚硫酸盐的葡萄糖产率最高(81.2%，以处理样品中的纤维素计)，比原料的葡萄糖产率(70.6%)高出10%以上。亚硫酸盐处理过程中产生了较多的磺酸基和弱酸性基团。同时，在所有亚硫酸盐处理的样品中，乙醇亚硫酸盐处理的样品具有最高的磺酸基(0.103 mmoL/g)和弱酸性基团(1.85 mmoL/g)。红外光谱中1168和1190 cm~(-1)谱带的变化证实了亚硫酸盐处理过程中木质素的磺化作用。在1458 cm-1处的谱带消失，表明亚硫酸盐处理过程中木质素上的甲氧基已被去除。结论亚硫酸盐处理可以使CCR中的木质素发生降解和磺化。由于磺酸基和弱酸基的增加，前处理改善了样品的亲水性，从而提高了材料的葡萄糖产率。乙醇亚硫酸盐预处理法脱除木质素效果最好，产糖率最高。
BackgroundCorn cob residue (CCR) is a kind of waste lignocellulosic material with enormous potential for bioethanol production. The moderated sulphite processes were used to enhance the hydrophily of the material by sulfonation and hydrolysis. The composition, FT-IR spectra, and conductometric titrations of the pretreated materials were measured to characterize variations of the CCR in different sulfite pretreated environments. And the objective of this study is to compare the saccharification rate and yield of the samples caused by these variations.ResultsIt was found that the lignin in the CCR (43.2%) had reduced to 37.8%, 38.0%, 35.9%, and 35.5% after the sulfite pretreatment in neutral, acidic, alkaline, and ethanol environments, respectively. The sulfite pretreatments enhanced the glucose yield of the CCR. Moreover, the ethanol sulfite sample had the highest glucose yield (81.2%, based on the cellulose in the treated sample) among the saccharification samples, which was over 10% higher than that of the raw material (70.6%). More sulfonic groups and weak acid groups were produced during the sulfite pretreatments. Meanwhile, the ethanol sulfite treated sample had the highest sulfonic group (0.103 mmol/g) and weak acid groups (1.85 mmol/g) in all sulfite treated samples. In FT-IR spectra, the variation of bands at 1168 and 1190 cm-1confirmed lignin sulfonation during sulfite pretreatment. The disappearance of the band at 1458 cm-1implied the methoxyl on lignin had been removed during the sulfite pretreatments.ConclusionsIt can be concluded that the lignin in the CCR can be degraded and sulfonated during the sulfite pretreatments. The pretreatments improve the hydrophility of the samples because of the increase in sulfonic group and weak acid groups, which enhances the glucose yield of the material. The ethanol sulfite pretreatment is the best method for lignin removal and with the highest glucose yield.
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