The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation
The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation
复制标题
DOI：
10.1128/iai.67.10.5427-5433.1999
复制
发表时间：
1999-10-01
影响因子：
3.1
通讯作者：
Götz, F
中科院分区：
文献类型：
--
作者：
Cramton, SE;Gerke, C;Götz, F
导致生物医学植入物表面上形成生物膜的医院感染是败血症的主要原因，并且通常与表皮葡萄球菌对植入物的定殖有关。生物膜的形成被认为需要两个连续的步骤：细胞粘附到固体基质上，然后是细胞-细胞粘附，产生多层细胞。细胞间粘附需要多糖细胞间粘附素（PIA），其由线性β-1，6-连接的葡糖胺聚糖组成，并且可以由细胞间粘附（伊卡）位点的UDP-N-乙酰葡糖胺副产物体外合成。我们研究了多种金黄色葡萄球菌菌株，发现所有测试菌株都含有伊卡位点，并且有几种菌株可以在体外形成生物膜。与S.伊卡基因显示59 - 78%的氨基酸同一性。伊卡基因座的缺失导致形成生物膜、产生PIA或介导体外N-乙酰葡糖胺基转移酶活性的能力丧失。跨物种杂交实验揭示了在其他几种葡萄球菌中存在icaA，这表明细胞-细胞粘附和形成生物膜的潜力在该属中是保守的。
Nosocomial infections that result in the formation of biofilms on the surfaces of biomedical implants are a leading cause of sepsis and are often associated with colonization of the implants by Staphylococcus epidermidis. Biofilm formation is thought to require two sequential steps: adhesion of cells to a solid substrate followed by cell-cell adhesion, creating multiple layers of cells. Intercellular adhesion requires the polysaccharide intercellular adhesin (PIA), which is composed of linear beta-1,6-linked glucosaminylglycans and can be synthesized in vitro from UDP-N-acetylglucosamine by products of the intercellular adhesion (ica) locus. We have investigated a variety of Staphylococcus aureus strains and find that all strains tested contain the ica locus and that several can form biofilms in vitro. Sequence comparison with the S. epidermidis ica genes revealed 59 to 78% amino acid identity. Deletion of the ica locus results in a loss of the ability to form biofilms, produce PIA, or mediate N-acetylglucosaminyltransferase activity in vitro. Cross-species hybridization experiments revealed the presence of icaA in several other Staphylococcus species, suggesting that cell-cell adhesion and the potential to form biofilms is conserved within this genus.