Two yeast PUF proteins negatively regulate a single mRNA
Two yeast PUF proteins negatively regulate a single mRNA
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DOI：
10.1074/jbc.m611253200
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发表时间：
2007-05-25
影响因子：
4.8
通讯作者：
Wickens, Marvin
中科院分区：
文献类型：
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作者：
Hook, Brad A.;Goldstrohm, Aaron C.;Wickens, Marvin
mRNA的稳定性和翻译受结合3 '-非翻译区的蛋白质阻遏物调节。PUF蛋白为这些调节分子提供了一个范例：像其他阻遏物一样，它们抑制翻译，增强mRNA衰变，并促进poly（A）去除。在这里，我们表明，一个单一的mRNA在酿酒酵母，编码HO内切酶，是由两个不同的PUF蛋白，Puf 4p和Mpt 5 p。这些蛋白质与相邻位点结合，可以共同占据mRNA。这两种蛋白质都是体内完全抑制和去腺苷化所必需的;它们的去除显着稳定了mRNA。这两种蛋白通过重叠但不相同的机制起作用：Puf 4p的抑制依赖于去腺苷化，而Mpt 5 p的抑制可以通过其他机制发生。两种调节蛋白的组合作用可能允许对特定环境线索的反应，并且在3 '-非翻译区介导的控制中是常见的。
mRNA stability and translation are regulated by protein repressors that bind 3'-untranslated regions. PUF proteins provide a paradigm for these regulatory molecules: like other repressors, they inhibit translation, enhance mRNA decay, and promote poly( A) removal. Here we show that a single mRNA in Saccharomyces cerevisiae, encoding the HO endonuclease, is regulated by two distinct PUF proteins, Puf4p and Mpt5p. These proteins bind to adjacent sites and can co-occupy the mRNA. Both proteins are required for full repression and deadenylation in vivo; their removal dramatically stabilizes the mRNA. The two proteins act through overlapping but non-identical mechanisms: repression by Puf4p is dependent on deadenylation, whereas repression by Mpt5p can occur through additional mechanisms. Combinatorial action of the two regulatory proteins may allow responses to specific environmental cues and be common in 3'-untranslated region-mediated control.