Cell type-specific chromatin structure determines the targeting of V(D)J recombinase activity in vitro
Cell type-specific chromatin structure determines the targeting of V(D)J recombinase activity in vitro
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DOI：
10.1016/s0092-8674(00)81272-6
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发表时间：
1996-06-14
期刊：
影响因子：
64.5
通讯作者：
Schlissel, MS
中科院分区：
文献类型：
--
作者：
StanhopeBaker, P;Hudson, KM;Schlissel, MS
一种识别保守重组信号序列（RSS）的常见V（D）J重组酶介导免疫球蛋白（IG）和T细胞受体（TCR）基因在B和T细胞前体中的组装。然而，特定IG和TCR基因片段的重排相对于细胞谱系和发育阶段受到严格调控。使用体外系统，我们分析了重组酶切割的RSSs侧翼的IG和TCR基因片段在细胞核中。我们发现，无论是谱系特异性和时间顺序的基因重排是反映在可访问性的RSS染色质内的体外切割。
A common V(D)J recombinase that recognizes a conserved recombination signal sequence (RSS) mediates the assembly of immunoglobulin (Ig) and T cell receptor (TCR) genes in B and T cell precursors. The rearrangement of particular Ig and TCR gene segments, however, is tightly regulated with respect to cell lineage and developmental stage. Using an in vitro system, we analyzed recombinase cleavage of RSSs flanking Ig and TCR gene segments in nuclei. We found that both the lineage-specificity and temporal ordering of gene rearrangement is reflected in the accessibility of RSSs within chromatin to in vitro cleavage.